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Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato
(Nature Publishing Group, 2021-02-22)DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein ... -
Characterization of a deletion in the Hsp70 cluster in the bovine reference genome
(Wiley Online Library, 2017-07-11)The 70 kilodalton heat shock proteins (Hsp70) are highly conserved molecular chaperones which have a crucial role in the stress response of the cell. In mammals, the Hsp70 proteins are encoded by a cluster of three genes: ... -
Effects of photoperiod sensitivity genes Ppd‐B1 and Ppd‐D1 on spike fertility and related traits in bread wheat
(Wiley Online Library, 2018-04-27)Increasing grain yield is a key breeding goal in bread wheat. Several authors have suggested that a spike fertility index (SF), that is the quotient between grain number per unit spike (GNS) and spikechaff dry weight (SCDW), ... -
Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos
(Plos One, 2014-09)Reproductive biotechnologies such as in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improved reproductive efficiency of animals. However, the birth rate of in vitro-derived embryos still lags ... -
Genome-Wide Analyses of Subtilisin-Like Serine Proteases on Solanum tuberosum
(Springer Netherlands, 2016-10)Plant subtilisin-like serine proteases or subtilases constitute a large expanded gene subfamily in potato. We identified 82 potato subtilases encoded by 74 genes. All subtilases present a characteristic S08 domain, and the ... -
Identificación y caracterización de Aspartil proteasas en Solanum tuberosum relacionadas a diferentes tipos de estrés
(Secretaria de Ciencia y Tecnología, Universidad Nacional de Mar del Plata, 2020-10)En este trabajo, realizamos un análisis del genoma de S. tuberosum para explorar las funciones potenciales de las Aspartil proteasas (AP, EC 3.4.23). Las APs son enzimas proteolíticas con funciones muy diversas que se ...