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    • Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato 

      Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Anderson, Mariette; Decima Oneto, Cecilia Andrea; Turesson, Helle; Storani, Leonardo; Olsson, Niklas; Fält, Ann Sofie; Hofvander, Per; Feingold, Sergio Enrique (Keystone Symposia on Molecular and Cellular Biology, 2021-03)
      StPPO2 gene editing was analyzed by Agrobacterium mediated transformation with CR-PPO vector, ribonucleoprotein complexes (RNP-PPO) transfection to protoplasts, and CR-PPO transient expression in protoplasts, yielding ...

    • Comparative potato genome editing: Agrobacterium tumefaciens-mediated transformation and protoplasts transfection delivery of CRISPR/Cas9 components directed to StPPO2 gene 

      Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Anderson, Mariette; Decima Oneto, Cecilia Andrea; Turesson, Helle; Olsson, Niklas; Fält, Ann Sofie; Hofvander, Per; Feingold, Sergio Enrique (Springer Netherlands, 2021-01-25)
      Delivery of the CRISPR/Cas9 components to the plant cells is a key step in its application as a genome editing tool. Here, we compared Agrobacterium-mediated transformation and protoplast transfection with CRISPR/Cas9 ...

    • CRISPR/Cas9 for potato functional genomics and breeding 

      Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Andersson, Mariette; Storani, Leonardo; Olsson, Niklas; Decima Oneto, Cecilia Andrea; Hofvander, Per; Feingold, Sergio Enrique (Humana Press (Springer), 2023-03-31)
      Cultivated potato (Solanum tuberosum L.) is one of the most important staple food crops worldwide. Its tetraploid and highly heterozygous nature pose a great challenge to its basic research and trait improvement through ...

    • Edición génica con el sistema CRISPR/Cas9: historia de su descubrimiento y alcances en agricultura 

      Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Feingold, Sergio Enrique (Universidad de Los Andes, Venezuela, 2021-04)
      La conversión del sistema inmune procariota CRISPR/Cas9 en una herramienta molecular para edición génica representa el avance tecnológico más destacado de la última década, y ha revolucionado tanto la investigación básica ...