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Mostrando ítems 1-15 de 15
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25S–18S rDNA IGS of Capsicum: molecular structure and comparison
Grabiele, Mauro; Debat, Humberto Julio; Moscone, Eduardo Alberto; Ducasse, Daniel Adrian (Springer, 2012-02)The primary and secondary structures of the intergenic spacer (IGS) between the 3′-end of 25S ribosomal RNA (rRNA) gene and the 5′-end of 18S rRNA gene are described for the cultivated chili pepper Capsicum pubescens. The ... -
A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae)
Aguilera, Patricia Mabel; Debat, Humberto Julio; Seijo, José Guillermo; Grabiele, Mauro (Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, 2021)Capsicum pubescens is a cultivated hot chili pepper, consumed in Latin American cuisine as a distinctive ingredient, and popularly known as “locoto” or “rocoto”. This taxon is also an outstanding source of resistance to ... -
An Integrated Physical Map of the Cultivated Hot Chili Pepper, Capsicum baccatum var. Pendulum
Aguilera, Patricia Mabel; Debat, Humberto Julio; Grabiele, Mauro (2017)Capsicum baccatum var. Pendulum is the most frequently cultivated chili pepper in South America. This taxon is an outstanding source for breeding programs and genetic improvement in the genus, since its traits can be ... -
Caracterización morfológica y cromosómica de Commelina Benghalensisl. (Commelinaceae ) de Argentina
Grabiele, Mauro; Honfi, Ana I.; Grabiele, Marina; Debat, Humberto Julio; Daviña, Julio R. (Ediciones de la Universidad de Concepción, Chile, 2009)Commelina benghalensis es una hierba pequeña, nativa de Africa y Asia tropical, aunque en la actualidad crece en casi todo el mundo. En América se la reporta creciendo en campo abierto, borde de bosques y áreas cultivadas ... -
The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.)
Debat, Humberto Julio; Grabiele, Mauro; Aguilera, Patricia Mabel; Bubillo, Rosana Elizabeth; Zapata, Pedro Darío; Marti, Dardo Andrea; Ducasse, Daniel Adrian (2014-10)We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, ... -
Dataset of the first transcriptome assembly of the tree crop “yerba mate” (Ilex paraguariensis) and systematic characterization of protein coding genes
Aguilera, Patricia Mabel; Debat, Humberto Julio; Grabiele, Mauro (2018-04)This contribution contains data associated to the research article entitled “Exploring the genes of yerba mate (Ilex paraguariensis A. St.-Hil.) by NGS and de novo transcriptome assembly” (Debat et al., 2014) [1]. By means ... -
Dataset of the transcribed 45S ribosomal RNA sequence of the tree crop “yerba mate”
Aguilera, Patricia Mabel; Debat, Humberto Julio; Grabiele, Mauro (2017-06)This contribution contains data related to the research article entitled “The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)” (Aguilera et al., 2016) [1]. Through a bioinformatic approach involving ... -
Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)
Scaldaferro, Marisel Analía; Grabiele, Mauro; Seijo, Jose Guillermo; Debat, Humberto Julio; Romero, María Victoria; Ducasse, Daniel Adrian; Prina, Alberto Raul; Moscone, Eduardo Alberto (2014)Purpose: To locate transient chromosome aberrations on a selected pepper cultivar and determine the tracing efficiency of different cytogenetic methods. Materials and methods: Seeds from Capsicum baccatum var. pendulum ... -
Exploring the genes of Yerba Mate (Ilex paraguariensis A. St.-Hil.) by NGS and De Novo Transcriptome Assembly
Debat, Humberto Julio; Grabiele, Mauro; Aguilera, Patricia Mabel; Bubillo, Rosana Elizabeth; Otegui, Mónica B.; Ducasse, Daniel Adrian; Zapata, Pedro Darío; Marti, Dardo Andrea (2014-10-16)Yerba mate (Ilex paraguariensis A. St.-Hil.) is an important subtropical tree crop cultivated on 326,000 ha in Argentina, Brazil and Paraguay, with a total yield production of more than 1,000,000 t. Yerba mate presents a ... -
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
Aguilera, Patricia Mabel; Debat, Humberto Julio; Scaldaferro, Marisel Analia; Marti, Dardo Andrea; Grabiele, Mauro (2016)We present here the physical mapping of the 5S rDNA locus in six wild and fi ve cultivated taxa of Capsicum by means of a genus-specifi c FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped ... -
Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization
Grabiele, Mauro; Debat, Humberto Julio; Scaldaferro, Marisel Analía; Aguilera, Patricia Mabel; Moscone, Eduardo Alberto; Seijo, José Guillermo; Ducasse, Daniel Adrian (Elsevier, 2018-08)Capsicum comprises 35 species of chili peppers and five of them are cultivated worldwide as spices or vegetables. Diploid karyotypes based in x = 12 and x = 13 are common in Capsicum and the constitutive heterochromatin ... -
Molecular characterization of the 5S rDNA non-transcribed spacer and reconstruction of phylogenetic relationships in Capsicum
Grabiele, Mauro; Aguilera, Patricia Mabel; Ducasse, Daniel Adrian; Debat, Humberto Julio (Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, 2021)Capsicum includes ca. 41 species of chili peppers. In this original report we PCR amplified, cloned, sequenced and characterized the 5S rDNA non-transcribed spacer -NTS- in 23 taxa of nine clades of Capsicum, divergent at ... -
The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
Aguilera, Patricia Mabel; Grabiele, Mauro; Debat, Humberto Julio; Bubillo, Rosana Elizabeth; Martí, Dardo Andrea (2016)We present the first report of the transcribed 18S–25S ribosomal DNA (rDNA) unit of the South American crop Ilex paraguariensis. This tree is consumed as an infusion named “mate”. Next-generation sequencing data were ... -
The DNAJ gene family in yerba mate (Ilex paraguariensis): genome-wide identification, structural characterization, orthology based classification and expression analysis
Aguilera, Patricia Mabel; Debat, Humberto Julio; Castrillo, María Lorena; Bich, Gustavo Angel; Grabiele, Mauro (Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, 2023-05-08)Dry leaves and twigs of yerba mate are widely infusion-consumed in southern Southamerica. Endemic and adapted to the Atlantic Forest, its extensive full-sun monoculture links to diverse biotic (pest, pathogens) and abiotic ... -
Use of silencing reporter and agroinfiltration transient assays to evaluate the potential of hpRNA construct to induce multiple tospovirus resistance
Debat, Humberto Julio; Grabiele, Mauro; Ducasse, Daniel Adrian; Lopez Lambertini, Paola Maria (2015-12)Tospoviruses are devastating plant viruses causing severe economic losses in a diverse range of crops worldwide. Here, we describe the development and evaluation of an RNA interference (RNAi) broad-spectrum virus resistance ...