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A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers
Llauger, Gabriela; Melero, Roberto; Monti, Demian Esteban; Sycz, Gabriela; Huck-Iriart, Cristian; Cerutti, Maria Laura; Klinke, Sebastián; Mikkelsen, Evelyn; Tijman, Ariel; Arranz, Rocío; Alfonso, Victoria; Arellano, Sofía Maité; Goldbaum, Fernando Alberto; Sterckx, Yann G. J.; Carazo, José María; Kaufman, Sergio B.; Dans, Pablo D.; Del Vas, Mariana; Otero, Lisandro H. (American Society for Microbiology, 2023-02)Fijiviruses replicate and package their genomes within viroplasms in a pro cess involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein ... -
EcXyl43 β-xylosidase: molecular modeling, activity on natural and artificial substrates, and synergism with endoxylanases for lignocellulose deconstruction
Ontañon, Ornella Mailén; Ghio, Silvina; Marrero Diaz De Vill, Rubén; Piccinni, Florencia Elizabeth; Talia, Paola Mónica; Cerutti, Maria Laura; Campos, Eleonora (Springer, 2018-08)Biomass hydrolysis constitutes a bottleneck for the biotransformation of lignocellulosic residues into bioethanol and high-value products. The efficient deconstruction of polysaccharides to fermentable sugars requires ...