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    • An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress 

      Gomez Mansur, Nabila M.; Pena, Liliana Beatriz; Bossio, Adrian Ezequiel; Lewi, Dalia Marcela; Beznec, Ailin; Blumwald, Eduardo; Arbona, Vicent; Gómez-Cadenas, Aurelio; Benavides, María Patricia; Gallego, Susana Mabel (Wiley, 2021-09)
      Cadmium is one of the most important contaminants and it induces severe plant growth restriction. In this study, we analyzed the metabolic changes associated with root growth restriction caused by cadmium in the early ...

    • CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa 

      Galindo Sotomonte, Luisa Fernanda; Jozefkowicz, Cintia; Gomez, Maria Cristina; Stritzler, Margarita; Frare, Romina Alejandra; Bottero, Ana Emilia; Tajima, Hiromi; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2023-02)
      Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying ...

    • Efficient CRISPR/Cas9 genome editing in alfalfa using a public germplasm 

      Bottero, Ana Emilia; Massa, Gabriela Alejandra; Gonzalez, Matías Nicolás; Stritzler, Margarita; Tajima, Hiromi; Gomez, Maria Cristina; Frare, Romina Alejandra; Feingold, Sergio Enrique; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Frontiers Media, 2021-07)
      Because its ability to acquire large amounts of nitrogen by symbiosis, tetraploid alfalfa is the main source of vegetable proteins in meat and milk production systems in temperate regions. Alfalfa cultivation also adds ...

    • Generation of a multi‑herbicide‑tolerant alfalfa by using base editing 

      Bottero, Ana Emilia; Gomez, Maria Cristina; Stritzler, Margarita; Tajima, Hiromi; Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-02)
      We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important ...

    • Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing 

      Stritzler, Margarita; Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Gomez, Maria Cristina; Frare, Romina Alejandra; Puebla, Andrea Fabiana; Tajima, Hiromi; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)
      The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ...

    • Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis 

      Frare, Romina Alejandra; Stritzler, Margarita; Gomez, Maria Cristina; Tajima, Hiromi; Pascuan, Cecilia Gabriela; López Fernández, María Paula; Bottero, Ana Emilia; Nikel, Pablo I.; Alleva, Karina Edith; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)
      The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities ...

    • Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought 

      Beznec, Ailin; Faccio, Paula Daniela; Miralles, Daniel J.; Abeledo, Leonor Gabriela; Decima Oneto, Cecilia Andrea; Garibotto, María De Belén; Gonzalez, Germán Andrés; Moreyra, Federico; Elizondo, Matias; Ruiz, Monica Beatriz; Lewi, Dalia Marcela; Blumwald, Eduardo; Llorente, Berta; Diaz Paleo, Antonio Horacio; Bossio, Adrian Ezequiel (Springer, 2021-05)
      Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, ...

    • Validation of housekeeping genes for qPCR in maize during water deficit stress conditions at flowering time 

      Decima Oneto, Cecilia Andrea; Bossio, Adrian Ezequiel; Faccio, Paula Daniela; Beznec, Ailin; Blumwald, Eduardo; Lewi, Dalia Marcela (2017)
      Plant stress studies are increasingly being based on gene expression. The analysis of gene expression requires sensitive, precise, and replicable measurements for specific mRNA sequences. Real-time RT-PCR is nowadays the ...

    • Water deficit stress tolerance in maize conferred by expression of an anisopentenyltransferase (IPT) gene driven by a stress- and maturation-induced promoter 

      Decima Oneto, Cecilia Andrea; Otegui, Marí­a Elena; Baroli, Irene; Beznec, Ailin; Faccio, Paula Daniela; Bossio, Adrian Ezequiel; Blumwald, Eduardo; Lewi, Dalia Marcela (2016)
      Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity ...