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A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers
Llauger, Gabriela; Melero, Roberto; Monti, Demian Esteban; Sycz, Gabriela; Huck-Iriart, Cristian; Cerutti, Maria Laura; Klinke, Sebastián; Mikkelsen, Evelyn; Tijman, Ariel; Arranz, Rocío; Alfonso, Victoria; Arellano, Sofía Maité; Goldbaum, Fernando Alberto; Sterckx, Yann G. J.; Carazo, José María; Kaufman, Sergio B.; Dans, Pablo D.; Del Vas, Mariana; Otero, Lisandro H. (American Society for Microbiology, 2023-02)Fijiviruses replicate and package their genomes within viroplasms in a pro cess involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein ... -
Development of nanobodies against Mal de Río Cuarto virus major viroplasm protein P9‑1 for diagnostic sandwich ELISA and immunodetection
Llauger, Gabriela; Monti, Demian Esteban; Aduriz Guerrero, Matías; Romão, Ema; Dumon, Analia Delina; Mattio, Maria Fernanda; Wigdorovitz, Andres; Muyldermans, Serge; Vincke, Cécile; Parreño, Viviana; Del Vas, Mariana (Springer Nature, 2021-10)Mal de Río Cuarto virus (MRCV) is a member of the genus Fijivirus of the family Reoviridae that causes a devastating disease in maize and is persistently and propagatively transmitted by planthopper vectors. Virus replication ...