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Resumen
The highly prevalent pest Alphitobius diaperinus (Coleoptera: Tenebrionidae) causes significant structural damage in poultry farms. Despite previous investigations on its carriage of pathogenic microorganisms, our understanding of its microbiome remains limited. This study aimed to analyze the diversity of culturable gut microbiota in A. diaperinus obtained from laboratory breeding. Fifteen seventh instar larvae underwent a 24-hour starvation period,
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dc.contributor.author | Antonuccio, Gisele Ivonne | |
dc.contributor.author | Sauka, Diego Hernan | |
dc.date.accessioned | 2024-02-14T10:46:22Z | |
dc.date.available | 2024-02-14T10:46:22Z | |
dc.date.issued | 2023-11-30 | |
dc.identifier.citation | 2nd International Electronic Conference on Microbiology (Noviembre 2023) | es_AR |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/16587 | |
dc.identifier.uri | https://sciforum.net/paper/view/16465 | |
dc.description | Poster y artículo | |
dc.description.abstract | The highly prevalent pest Alphitobius diaperinus (Coleoptera: Tenebrionidae) causes significant structural damage in poultry farms. Despite previous investigations on its carriage of pathogenic microorganisms, our understanding of its microbiome remains limited. This study aimed to analyze the diversity of culturable gut microbiota in A. diaperinus obtained from laboratory breeding. Fifteen seventh instar larvae underwent a 24-hour starvation period, followed by surface disinfection. Dissected midguts were homogenized and plated on nutrient agar (NA), brain heart infusion agar (BHI), and Bacillus cereus agar (BC). The cultured isolates were subjected to gram staining, phylogenetic analysis, biochemical property evaluation, and metabolic activity assessment. Bacterial counts were higher in BHI (2.51x105 CFU/gut) than in NA (2.25x105 CFU/gut), possibly due to nutrient richness. NA exhibited a dominant colony morphology of gram-negative bacilli, while BHI displayed additional distinct colonies of gram-positive cocci. Surprisingly, yeast-like colonies were observed on BC plates. Based on 16S rRNA gene sequences, eight bacterial isolates were identified as Enterobacter sp., and two as Staphylococcus sp. Using RNA gene ITS region sequences, two yeast isolates were identified as Debaryomyces sp. and Hyphopichia sp. A preliminary species-level identification of bacteria (Enterobacter cloacae, Staphylococcus gallinarum, and Staphylococcus succinus) was achieved using API systems and complementary biochemical tests. Discrepancies between phylogenetic analysis and phenotypic data suggest the potential existence of new species or subspecies. Further comprehensive studies are required to confirm this hypothesis. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Sciforum | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | es_AR |
dc.source | 2nd International Electronic Conference on Microbiology (Noviembre 2023) | es_AR |
dc.subject | Control Biológico | es_AR |
dc.subject | Biological Control | eng |
dc.subject | Gusano de la Harina | |
dc.subject | Yellow Mealworm | eng |
dc.subject | Alphitobius diaperinus | |
dc.subject | Flora Microbiana | |
dc.subject | Microbial Flora | eng |
dc.subject.other | Microbiota | eng |
dc.subject.other | Culturable Microorganisms | eng |
dc.subject.other | Bacterial Diversity | eng |
dc.title | Isolation and identification of culturable gut microbiota in the larval stage of lesser mealworm (Alphitobius diaperinus) | es_AR |
dc.type | info:ar-repo/semantics/documento de conferencia | es_AR |
dc.type | info:eu-repo/semantics/conferenceObject | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | es_AR |
dc.description.origen | Instituto de Microbiología y Zoología Agrícola (IMYZA) | es_AR |
dc.description.fil | Fil: Antonuccio, Gisele Ivonne. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina | es_AR |
dc.description.fil | Fil: Antonuccio, Gisele Ivonne. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina | es_AR |
dc.description.fil | Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina | es_AR |
dc.description.fil | Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | ponencia |
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