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Diagnósticos certeros como base de un manejo adecuado: herramientas actuales al alcance del sector productivo
(IPAVE - CIAP, INTA; Universidad Nacional de Córdoba - Facultad de Ciencias Agropecuarias FCA, 2024-04-12)Diagnósticos certeros como base de un manejo adecuado:Herramientas actuales al alcance del sector productivo ¿QUÉ ES DIAGNOSTICAR? RAE: Determinar el carácter de una enfermedad mediante el examen de sus signos. La pregunta ... -
Diferenciación de serovares de leptospiras patógenas mediante PCR del gen ligB y secuenciación = Differentiation of pathogenic leptospires spp by PCR of ligB gene and sequencing
(Asociación Argentina de Microbiología, 2018)La leptospirosis es la zoonosis de mayor distribución mundial. El objetivo del presente trabajo fue desarrollar una técnica molecular que diferencie leptospiras patógenas. Se amplificó mediante PCR y se secuenció una ... -
Early detection of Ascochyta blight (Ascochyta rabiei) of chickpea by traditional PCR
(Elsevier, 2020-11-16)Ascochyta blight is the major disease affecting chickpea (Cicer arietinum) around the world. Since the first report of Ascochyta rabiei's isolation in Argentina in 2012, the pathogen has caused severe economic losses in ... -
Effect of the addition of phytosterols and tocopherols on Streptococcus thermophilus robustness during industrial manufacture and ripening of a functional cheese as evaluated by qPCR and RT-qPCR
(2016-09)The quality of functional food products designed for the prevention of degenerative diseases can be affected by the incorporation of bioactive compounds. In many types of cheese, the performance of starter microorganisms ... -
First report of pathogenic Leptospira spp. in Tadarida brasiliensis bats (family Molossidae) and Eptesicus furinalis (family Vespertilionidae) of Argentina. New host species in this country? = Primer reporte de Leptospira spp. patógena en murciélagos Tadarida brasiliensis (familia: Molossidae) y Eptesicus furinalis (familia: Vespertilionidae) en Argentina. ¿Nuevas especies hospedadoras en el país?
(Elsevier, 2021-09)Leptospirosis is an endemic disease caused by Leptospira spp., a bacterium that affects animals and humans. In recent years, the number of reports of leptospirosis in wild animals has increased, which highlights the need ... -
First report of rice stripe necrosis virus in Argentina
(Elsevier, 2018-12)Since 2008/2009, we have seen symptoms of viral infection in rice plants from crop regions in Argentina. The main objective of this work was to identify the causal agent of the rice plants' crinkling. We therefore analyzed ... -
Generalized glycogenosis in Brahman-derived breeds: diagnosis and prevalence in Argentina
(Springer, 2019-08)Generalized glycogenosis is a lethal autosomal recessive disease caused by a deficient activity of the acidic 1,4-α-glucosidase enzyme and characterized by an accumulation of glycogen within lysosomes. Three mutations in ... -
Genotipificación de variantes de beta-caseína bovina mediante ACRSPCR- RFLP = Genotyping of bovine beta-casein variants by ACRS-PCR-RFLP
(Consejo de Rectores de Universidades Privadas, 2023-10)Las variantes de la beta-caseína bovina pueden clasificarse en dos grupos, tipo-A1 y tipo-A2, según el aminoácido que esté presente en la posición 67 de la proteína (His, tipo A1; Pro, tipo A2)1. En los últimos años se ha ... -
Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina
(Wiley, 2020-07)Bovine babesiosis is a tick‐transmitted haemoparasitic disease caused by Babesia bovis and B. bigemina affecting cattle of tropical and subtropical regions around the world. Pathogens are transmitted by the tick vector ... -
Impact of extracellular nucleic acids from lactic acid bacteria on qPCR and RT-qPCR results in dairy matrices : implications for defining molecular markers of cell integrity
(2017-07)In this study, we evaluated whether quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) results could be affected by extracellular (free) DNA or RNA, respectively, obtained either from isolated bacteria or ... -
Incidence and prevalence of aphid-borne viruses infecting strawberry in Argentina
(Wiley, 2018-07)The incidence and prevalence of strawberry viruses were determined in surveys of randomly selected strawberry plants grown in different regions of Argentina. In 2009 and 2010, 1034 plants from 28 fields and 1060 plants ... -
International interlaboratory validation of a nested PCR for molecular detection of Babesia bovis and Babesia bigemina, causative agents of bovine babesiosis
(Elsevier, 2022-04)Babesia bovis and B. bigemina are tick-transmitted parasites causing bovine babesiosis, characterized by significant morbidity and mortality leading to economic losses to the livestock industry in tropical and subtropical ... -
L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus
(2019-11)Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive ... -
Manual sobre diagnóstico molecular de leptospirosis
(INTA Ediciones, 2022)Este manual es una recopilación sobre las herramientas moleculares de diagnóstico y caracterización de cepas aisladas. Este trabajo es resultado de la integración de más de 10 años de trabajo durante mi tesis doctoral y ... -
Microscopic and PCR-based detection of microsporidia spores in human stool samples = Detección microscópica y molecular de esporas de microsporidios en muestras de heces humanas
(Elsevier, 2020-06)Microsporidia are obligate intracellular fungi with a remarkable ability to infect a wide range of invertebrate and vertebrate hosts. Namely, Enterocytozoon bieneusi is the most frequently microsporidia reported worldwide, ... -
Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
(Elsevier, 2023-04)A growing interest in the production and commercialization of A2 cow's milk has been observed in many countries in the last few years due to the beneficial properties for human health attributed to A2 β-casein variant. ... -
Molecular diagnosis of Leishmania spp. in dogs of a subtropical locality of Argentina
(Wiley, 2020-07)Leishmaniosis is a tropical and subtropical vector‐borne disease caused by hemoparasites of the genus Leishmania. The disease can infect humans, as well as domestic and wildlife animals. Dogs are the main reservoir for L. ... -
Molecular sequence data of Thecaphora frezii affecting peanut crops in Argentina
(Springer, 2013-12)In Argentina, Thecaphora frezii is the causal agent of peanut smut, causing severe yield losses in peanut growing areas. In this work sequence data of the large ribosomal subunit (28S rDNA) of four T. frezii specimens from ... -
Multiplex PCR followed by restriction length polymorphism analysis for the subtyping of bovine herpesvirus 5 isolates
(BMC, 2013-06)Background: Several types and subtypes of bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, making type/subtype differentiation essential to understand the ... -
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
(Elsevier, 2018-07)Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are ...