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Abstract
Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of infection (LI) by assessing differences in proviral load in peripheral blood. This phenotypic variation appears to be determined by host genetics variants, especially those located in the BoLA-DRB3 MHCII molecule. We performed an [ver mas...]
dc.contributor.authorCarignano, Hugo Adrian
dc.contributor.authorBeribe, Maria Jose
dc.contributor.authorCaffaro, Marí­a Eugenia
dc.contributor.authorAmadio, Ariel
dc.contributor.authorNani, Juan Pablo
dc.contributor.authorGutierrez, Gerónimo
dc.contributor.authorAlvarez, Irene
dc.contributor.authorTrono, Karina Gabriela
dc.contributor.authorMiretti, Marcos Mateo
dc.contributor.authorPoli, Mario Andres
dc.date.accessioned2017-10-06T14:35:02Z
dc.date.available2017-10-06T14:35:02Z
dc.date.issued2017-05
dc.identifier.issn0268-9146 (Print)
dc.identifier.issn1365-2052 (Online)
dc.identifier.otherhttps://doi.org/10.1111/age.12566
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1430
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1111/age.12566/abstract;jsessionid=1A21B9D057520F287700321689693BE3.f04t02
dc.description.abstractBovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of infection (LI) by assessing differences in proviral load in peripheral blood. This phenotypic variation appears to be determined by host genetics variants, especially those located in the BoLA-DRB3 MHCII molecule. We performed an association study using sequencing-based typed BOLA-DRB3 alleles from over 800 Holstein and Holstein × Jersey cows considering LI in vivo and accounting for filial relationships. The DBR3*0902 allele was associated with a low level of infection (LLI) (<1% of circulating infected B-cells), whereas the DRB3*1001 and DRB3*1201 alleles were related to a high level of infection (HLI). We found evidence that 13 polymorphic positions located in the pockets of the peptide-binding cleft of the BOLA-DRB3 alleles were associated with LI. DRB3*0902 had unique haplotypes for each of the pockets: Ser13-Glu70-Arg71-Glu74 (pocket 4), Ser11-Ser30 (pocket 6), Glu28-Trp61-Arg71 (pocket 7) and Asn37-Asp57 (pocket 9), and all of them were significantly associated with LLI. Conversely, Lys13-Arg70-Ala71-Ala74 and Ser13-Arg70-Ala71-Ala74, corresponding to the DRB3*1001 and *1201 alleles respectively, were associated with HLI. We showed that the specific amino acid pattern in the DRB3*0902 peptide-binding cleft may be related to the set point of a very low proviral load level in adult cows. Moreover, we identified two BOLA-DRB3 alleles associated with a HLI, which is compatible with a highly contagious profile.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceAnimal genetics 48 (4) : 420–430. (August 2017)eng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectVirus Leucemia Bovina
dc.subjectBovine Leukaemia Viruseng
dc.subjectRazas (Animales)
dc.subjectBreeds (Animals)eng
dc.subjectCruzamiento
dc.subjectCrossbreedingeng
dc.subjectPolimorfismo
dc.subjectPolymorphismeng
dc.subjectGenética
dc.subjectGeneticseng
dc.subject.otherRaza Holstein
dc.subject.otherRaza Jersey
dc.titleBOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattleeng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/acceptedVersioneng
dc.description.filFil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
dc.description.filFil: Beribe, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
dc.description.filFil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
dc.description.filFil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Nani, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.filFil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Miretti, Marcos Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
dc.description.filFil: Poli, Mario AndresInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
dc.subtypecientifico


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