Mostrar el registro sencillo del ítem

resumen

Resumen
By amplification and sequencing of 18S rRNA gene fragments, Hepatozoon spp. DNA was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing Ixodes ricinus ticks from Slovakia and Czech Republic, respectively. Hepatozoon spp. DNA was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from Slovakia. Prevalence of infection was significantly higher in Myodes glareolus (11.45 %) than in Apodemus spp. (0.28 %) (P < 0.001). Sequencing of 18S [ver mas...]
dc.contributor.authorHamšíkova, Zuzana
dc.contributor.authorSilaghi, Cornelia
dc.contributor.authorRudolf, Ivo
dc.contributor.authorVenclíková, Kristýna
dc.contributor.authorMahríkova, Lenka
dc.contributor.authorSlovák, Mirko
dc.contributor.authorMendel, Jan
dc.contributor.authorBlažejová, Hana
dc.contributor.authorBerthová, Lenka
dc.contributor.authorKocianová, Elena
dc.contributor.authorHubálek, Zdeněk
dc.contributor.authorSchnittger, Leonhard
dc.contributor.authorKazimírová, Mária
dc.coverage.spatialSlovakia (nation)
dc.coverage.spatialCzech Republic (nation)
dc.date.accessioned2017-09-28T13:48:52Z
dc.date.available2017-09-28T13:48:52Z
dc.date.issued2016-10
dc.identifier.issn0932-0113 (Print)
dc.identifier.issn1432-1955 (Online)
dc.identifier.otherhttps://doi.org/10.1007/s00436-016-5156-5
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1347
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00436-016-5156-5
dc.description.abstractBy amplification and sequencing of 18S rRNA gene fragments, Hepatozoon spp. DNA was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing Ixodes ricinus ticks from Slovakia and Czech Republic, respectively. Hepatozoon spp. DNA was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from Slovakia. Prevalence of infection was significantly higher in Myodes glareolus (11.45 %) than in Apodemus spp. (0.28 %) (P < 0.001). Sequencing of 18S rRNA Hepatozoon spp. gene amplicons from I. ricinus showed 100 % identity with Hepatozoon canis isolates from red foxes or dogs in Europe. Phylogenetic analysis showed that at least two H. canis 18S rRNA genotypes exist in Slovakia of which one was identified also in the Czech Republic. The finding of H. canis in questing I. ricinus suggests the geographical spread of the parasite and a potential role of other ticks as its vectors in areas where Rhipicephalus sanguineus is not endemic. Sequencing of 18S rRNA gene amplicons from M. glareolus revealed the presence of two closely related genetic variants, Hepatozoon sp. SK1 and Hepatozoon sp. SK2, showing 99–100 % identity with isolates from M. glareolus from other European countries. Phylogenetic analysis demonstrates that 18S rRNA variants SK1 and SK2 correspond to previously described genotypes UR1 and UR2 of H. erhardovae, respectively. The isolate from Apodemus flavicollis (Hepatozoon sp. SK3b) was 99 % identical with isolates from reptiles in Africa and Asia. Further studies are necessary to identify the taxonomic status of Hepatozoon spp. parasitizing rodents in Europe and the host-parasite interactions in natural foci.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceParasitology research 115 (10) : 3897–3904. (October 2016)
dc.subjectIxodes Ricinuses_AR
dc.subjectMetastigmata
dc.subjectHepatozoon
dc.subjectSporozoa
dc.subjectRoedores
dc.subjectRodentseng
dc.subject.otherGarrapatas
dc.subject.otherApicomplexa
dc.subject.otherEslovaquia
dc.subject.otherRepública Checa
dc.titleMolecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republices_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/acceptedVersiones_AR
dc.description.filFil: Hamšíková, Zuzana. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
dc.description.filFil: Silaghi, Cornelia. Ludwig-Maximilians-Universität. Comparative Tropical Medicine and Parasitology; Alemania. University of Zurich. Institute of Parasitology. National Centre for Vector Entomology; Suiza
dc.description.filFil: Rudolf, Ivo. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa
dc.description.filFil: Venclíková, Kristýna. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa. The Czech Academy of Sciences. Institute of Macromolecular Chemistry; República Checa
dc.description.filFil: Mahrikova, Lenka. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
dc.description.filFil: Slovak, Mirko. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
dc.description.filFil: Mendel, Jan. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa.
dc.description.filFil: Blažejová, Hana. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa.
dc.description.filFil: Berthova, Lenka. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia
dc.description.filFil: Kocianova, Elena. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia
dc.description.filFil: Hubálek, Zdeněk. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa
dc.description.filFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Kazimirová, Mária. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
dc.subtypecientifico


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

common

Mostrar el registro sencillo del ítem