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Abstract
Bacillus thuringiensis is a Gram-positive and sporulated bacterium exhibiting insecticidal activity against a wide range of insects.3 During sporulation, this bacterium produces a number of different proteins forming crystalline inclusions adjacent to the spores (parasporal crystals). Among these insecticidal proteins, the most abundant are those commonly known as Cry (Crystal) proteins, which are responsible for exerting a toxic activity (upon ingestion) [ver mas...]
dc.contributor.authorPeralta, Cecilia
dc.contributor.authorSauka, Diego Herman
dc.contributor.authorMarozzi, Antonela Alejandra
dc.contributor.authordel Valle, Eleodoro Eduardo
dc.contributor.authorPalma, Leopoldo
dc.date.accessioned2022-07-25T10:33:19Z
dc.date.available2022-07-25T10:33:19Z
dc.date.issued2021-10
dc.identifier.issn0325-7541
dc.identifier.otherhttps://doi.org/10.1016/j.ram.2020.09.005
dc.identifier.urihttp://hdl.handle.net/20.500.12123/12391
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0325754120300924
dc.description.abstractBacillus thuringiensis is a Gram-positive and sporulated bacterium exhibiting insecticidal activity against a wide range of insects.3 During sporulation, this bacterium produces a number of different proteins forming crystalline inclusions adjacent to the spores (parasporal crystals). Among these insecticidal proteins, the most abundant are those commonly known as Cry (Crystal) proteins, which are responsible for exerting a toxic activity (upon ingestion) against insects of different species.5 For this reason, B. thuringiensis has proved to be the most efficient and used bioinsecticide to date.2 However, Spodoptera cosmioides, Spodoptera eridania and Agrotis sp. (Lepidoptera) are species that are not yet controlled by some transgenic crops (e.g. Intacta RR2Pro soybean). Thus, in an attempt to enlarge the host spectrum of this bacterium it is necessary to search for novel strains. In this work we show a sporulated B. thuringiensis Bt-UNVM_84 strain exhibiting a number of rare amorphous to spherical crystal combinations, whereas sporulated B. thuringiensis strain Bt-UNVM-94 showed quasi symmetric bipyramidal parasporal crystals, by using scanning electron microscopy (SEM) (Fig. 1). Strains Bt-UNVM_84 and Bt-UNVM_94 were isolated from Oncativo (Córdoba, Argentina) and Cululú (Santa Fe, Argentina), respectively. The insecticidal activity of these different B. thuringiensis strains is currently under investigation. Each strain was grown in liquid CCY sporulation medium6 for ∼48h (150rpm) until no vegetative cells were observed under a light microscope. The presence of parasporal crystals was first determined using Coomassie blue stained slides1 (1000×) under a Nikon E100 light microscope and confirmed later by a Nikon Ti-Eclipse phase contrast microscope (1000×) (data not shown). For the SEM analysis, aliquots of 1ml were centrifuged for 5minutes (16,000g) at room temperature. Each pellet was washed three times with sterile distilled water and fixed with 100μl 4% formaldehyde. Each fixed preparation was then sent to Centro Integral de Microscopía Electrónica (CIME – CONICET – UNT) for SEM examination.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherAsociación Argentina de Microbiologíaes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceRevista Argentina de Microbiología 53 (4) : 378-379 (Octubre - Diciembre 2021)es_AR
dc.subjectBacillus thuringiensises_AR
dc.subjectBacteriaes_AR
dc.subjectMorfología
dc.subjectMorphologyeng
dc.subjectArgentina
dc.titleArgentinean Bacillus thuringiensis strains exhibiting distinct morphology of their parasporal crystalses_AR
dc.title.alternativeCepas argentinas de Bacillus thuringiensis con distinta morfología en sus cristales paraesporaleses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Microbiología y Zoología Agrícola (IMYZA)es_AR
dc.description.filFil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentinaes_AR
dc.description.filFil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentinaes_AR
dc.description.filFil: Marozzi, Antonela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA). Universidad Nacional del Comahue (UNCo); Argentinaes_AR
dc.description.filFil: del Valle, Eleodoro Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentinaes_AR
dc.subtypecientifico


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