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Plant viruses encode movement proteins (MP) to facilitate cell-to-cell transport through plasmodesmata. In this study, using trans-complementation of a movement-defective turnip vein-clearing tobamovirus (TVCV) replicon, we show for the first time for cytorhabdoviruses (lettuce necrotic yellows virus (LNYV) and alfalfa dwarf virus (ADV)) that their P3 proteins function as MP similar to the TVCV P30 protein. All three MP localized to plasmodesmata when [ver mas...]
dc.contributor.authorMann, Krin S.
dc.contributor.authorBejerman, Nicolas Esteban
dc.contributor.authorJohnson, Karyn N.
dc.contributor.authorDietzgen, Ralf Georg
dc.date.accessioned2017-09-18T13:19:02Z
dc.date.available2017-09-18T13:19:02Z
dc.date.issued2016
dc.identifier.issn0042-6822
dc.identifier.otherhttps://doi.org/10.1016/j.virol.2015.11.028
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1235
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0042682215005097
dc.description.abstractPlant viruses encode movement proteins (MP) to facilitate cell-to-cell transport through plasmodesmata. In this study, using trans-complementation of a movement-defective turnip vein-clearing tobamovirus (TVCV) replicon, we show for the first time for cytorhabdoviruses (lettuce necrotic yellows virus (LNYV) and alfalfa dwarf virus (ADV)) that their P3 proteins function as MP similar to the TVCV P30 protein. All three MP localized to plasmodesmata when ectopically expressed. In addition, we show that these MP belong to the 30K superfamily since movement was inhibited by mutation of an aspartic acid residue in the critical 30K-specific LxD/N50–70G motif. We also report that Nicotiana benthamiana microtubule-associated VOZ1-like transcriptional activator interacts with LNYV P3 and TVCV P30 but not with ADV P3 or any of the MP point mutants. This host protein, which is known to interact with P3 of sonchus yellow net nucleorhabdovirus, may be involved in aiding the cell-to-cell movement of LNYV and TVCV.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceVirology 489 : 20-33. (February 2016)eng
dc.subjectEnfermedades de las Plantas
dc.subjectPlant Diseaseseng
dc.subjectVirus de las Plantas
dc.subjectPlant Viruseseng
dc.subjectProteinseng
dc.subjectProteínas
dc.subject.otherCytorhabdovirus
dc.titleCytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteinseng
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/acceptedVersioneng
dc.description.origenInst. Patología Vegetal
dc.gic152356
dc.description.filFil: Mann, Krin S. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
dc.description.filFil: Bejerman, Nicolás Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Johnson, Karyn N. University of Queensland. School of Biological Sciences; Australia
dc.description.filFil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
dc.subtypecientifico


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