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Abstract
Maize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing [ver mas...]
dc.contributor.authorCamiletti, Boris Xavier
dc.contributor.authorMoral, Juan
dc.contributor.authorAsensio, Claudia Mariana
dc.contributor.authorTorrico Ramallo, Ada Karina
dc.contributor.authorLucini, Enrique Iván
dc.contributor.authorGimenez, Maria De La Paz
dc.contributor.authorMichailides, Themis J.
dc.date.accessioned2022-06-06T10:19:51Z
dc.date.available2022-06-06T10:19:51Z
dc.date.issued2018-05-07
dc.identifier.issn0031-949X
dc.identifier.otherhttps://doi.org/10.1094/PHYTO-07-17-0255-R
dc.identifier.urihttp://hdl.handle.net/20.500.12123/12018
dc.identifier.urihttps://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-17-0255-R
dc.description.abstractMaize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing mycotoxins in order to select atoxigenic strains to be used as potential biocontrol agents (BCA). All of the isolates were tested for aflatoxin and cyclopiazonic acid (CPA) production in maize kernels and a liquid culture medium. Genetic diversity of the nonaflatoxigenic isolates was evaluated by analysis of vegetative compatibility groups (VCG) and confirmation of deletions in the aflatoxin biosynthesis cluster. Eight atoxigenic isolates were compared for their ability to reduce aflatoxin and CPA contamination in maize kernels in coinoculation tests. The A. flavus population was composed of 32% aflatoxin and CPA producers and 52% CPA producers, and 16% was determined as atoxigenic. All of the aflatoxin producer isolates also produced CPA. Aflatoxin and CPA production was significantly higher in maize kernels than in liquid medium. The 57 nonaflatoxigenic strains formed six VCG, with AM1 and AM5 being the dominant groups, with a frequency of 58 and 35%, respectively. In coinoculation experiments, all of the atoxigenic strains reduced aflatoxin from 54 to 83% and CPA from 60 to 97%. Members of group AM1 showed a greater aflatoxin reduction than members of AM5 (72 versus 66%) but no differences were detected in CPA production. Here, we described for the first time atoxigenic isolates of A. flavus that show promise to be used as BCA in maize crops in Argentina. This innovating biological control approach should be considered, developed further, and used by the maize industry to preserve the quality properties and food safety of maize kernels in Argentina.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherAmerican Phytopathological Societyes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourcePhytopathology 108 (7) : 818-828 (2018)es_AR
dc.subjectAspergillus Flavuses_AR
dc.subjectMaizeeng
dc.subjectZea Mayses_AR
dc.subjectMycotoxinseng
dc.subjectArgentinaes_AR
dc.subjectAflatoxinases_AR
dc.subjectMaízes_AR
dc.subjectMicotoxinases_AR
dc.titleCharacterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maizees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Camiletti, B.X. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.es_AR
dc.description.filFil: Camiletti, B.X. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentinaes_AR
dc.description.filFil: Moral, Juan. University of Córdoba. Campus de Rabanales. Departamento de Agronomía; Españaes_AR
dc.description.filFil: Moral, Juan. University of California. Kearney Agricultural Research and Extension Center; Estados Unidoses_AR
dc.description.filFil: Asensio, Claudia M. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Química Biológica; Argentinaes_AR
dc.description.filFil: Asensio, Claudia M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Lucini, Enrique I. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Microbiología Agrícola; Argentinaes_AR
dc.description.filFil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Michailides, Themis J. University of California. Kearney Agricultural Research and Extension Center; Estados Unidoses_AR
dc.subtypecientifico


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