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Resumen
Babesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B.
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dc.contributor.author | Gallenti, Romina Josefina | |
dc.contributor.author | Hussein, Hala E. | |
dc.contributor.author | Alzan, Heba F. | |
dc.contributor.author | Suarez, Carlos Esteban | |
dc.contributor.author | Ueti, Massaro W. | |
dc.contributor.author | Asurmendi, Sebastian | |
dc.contributor.author | Benitez, Daniel Francisco | |
dc.contributor.author | Araujo, Flabio Ribeiro de | |
dc.contributor.author | Rolls, Peter | |
dc.contributor.author | Sibeko, Kgomotso | |
dc.contributor.author | Schnittger, Leonhard | |
dc.contributor.author | Florin-Christensen, Mónica | |
dc.date.accessioned | 2022-05-16T16:34:13Z | |
dc.date.available | 2022-05-16T16:34:13Z | |
dc.date.issued | 2022-03 | |
dc.identifier.issn | 2076-0817 | |
dc.identifier.other | https://doi.org/10.3390/pathogens11030344 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/11886 | |
dc.identifier.uri | https://www.mdpi.com/2076-0817/11/3/344 | |
dc.description.abstract | Babesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. bovis contains five rom4 paralogous genes in chromosome 2, which we have named rom4.1, 4.2, 4.3, 4.4 and 4.5. There are moderate degrees of sequence identity between them, except for rom4.3 and 4.4, which are almost identical. RT-qPCR analysis showed that rom4.1 and rom4.3/4.4, respectively, display 18-fold and 218-fold significantly higher (p < 0.01) levels of transcription in SS than in AS, suggesting a role in gametogenesis-related processes. In contrast, transcription of rom4.4 and 4.5 differed non-significantly between the stages. ROM4 polymorphisms among geographic isolates were essentially restricted to the number of tandem repeats of a 29-amino acid sequence in ROM4.5. This sequence repeat is highly conserved and predicted as antigenic. B. bovis ROMs likely participate in relevant host–pathogen interactions and are possibly useful targets for the development of new control strategies against this pathogen. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | MDPI | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud” | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Pathogens 11 (3) : 344 (Marzo 2022) | es_AR |
dc.subject | Tick-borne Diseases | eng |
dc.subject | Enfermedades Transmitidas por Garrapatas | es_AR |
dc.subject | Babesiosis | eng |
dc.subject | Bovinae | es_AR |
dc.subject | Babesia bovis | es_AR |
dc.subject | Gene Expression | eng |
dc.subject | Expresión Génica | es_AR |
dc.subject | Polymorphism | eng |
dc.subject | Polimorfismo | es_AR |
dc.subject | Bioinformatics | eng |
dc.subject | Bioinformática | es_AR |
dc.subject.other | Experimental Studies | eng |
dc.subject.other | Estudios Experimentales | es_AR |
dc.subject.other | Rhomboid Serine Protease | eng |
dc.subject.other | Proteasa de Serina Romboidal | es_AR |
dc.title | Unraveling the complexity of the rhomboid serine protease 4 family of Babesia bovis using bioinformatics and experimental studies | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Patobiología | es_AR |
dc.description.fil | Fil: Gallenti, Romina Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina | es_AR |
dc.description.fil | Fil: Gallenti, Romina Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Hussein, Hala E. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos | es_AR |
dc.description.fil | Fil: Hussein, Hala E. Cairo University. Faculty of Science. Department of Entomology; Egipto | es_AR |
dc.description.fil | Fil: Alzan, Heba F. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidos | es_AR |
dc.description.fil | Fil: Alzan, Heba F. National Research Center. Tick and Tick-Borne Disease Research Unit; Egipto | es_AR |
dc.description.fil | Fil: Suarez, Carlos Esteban. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos | es_AR |
dc.description.fil | Fil: Suarez, Carlos Esteban. United States Department of Agricultural-Agricultural Research Service. Animal Disease Research Unit; Estados Unidos | es_AR |
dc.description.fil | Fil: Ueti, Massaro W. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos | es_AR |
dc.description.fil | Fil: Ueti, Massaro W. United States Department of Agricultural-Agricultural Research Service. Animal Disease Research Unit; Estados Unidos | es_AR |
dc.description.fil | Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Benitez, Daniel Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina | es_AR |
dc.description.fil | Fil: Araujo, Flabio R. EMBRAPA ; Brasil | es_AR |
dc.description.fil | Fil: Rolls, P. Tick Fever Centre. Department of Agriculture & Fisheries; Australia | es_AR |
dc.description.fil | Fil: Sibeko, Kgomotso. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases. Vectors and Vector-borne Diseases Research Programme; Sudáfrica | es_AR |
dc.description.fil | Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina | es_AR |
dc.description.fil | Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina | es_AR |
dc.description.fil | Fil: Florin-Christensen, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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