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Abstract
Babesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. [ver mas...]
dc.contributor.authorGallenti, Romina Josefina
dc.contributor.authorHussein, Hala E.
dc.contributor.authorAlzan, Heba F.
dc.contributor.authorSuarez, Carlos Esteban
dc.contributor.authorUeti, Massaro W.
dc.contributor.authorAsurmendi, Sebastian
dc.contributor.authorBenitez, Daniel Francisco
dc.contributor.authorAraujo, Flabio Ribeiro de
dc.contributor.authorRolls, Peter
dc.contributor.authorSibeko, Kgomotso
dc.contributor.authorSchnittger, Leonhard
dc.contributor.authorFlorin-Christensen, Mónica
dc.date.accessioned2022-05-16T16:34:13Z
dc.date.available2022-05-16T16:34:13Z
dc.date.issued2022-03
dc.identifier.issn2076-0817
dc.identifier.otherhttps://doi.org/10.3390/pathogens11030344
dc.identifier.urihttp://hdl.handle.net/20.500.12123/11886
dc.identifier.urihttps://www.mdpi.com/2076-0817/11/3/344
dc.description.abstractBabesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. bovis contains five rom4 paralogous genes in chromosome 2, which we have named rom4.1, 4.2, 4.3, 4.4 and 4.5. There are moderate degrees of sequence identity between them, except for rom4.3 and 4.4, which are almost identical. RT-qPCR analysis showed that rom4.1 and rom4.3/4.4, respectively, display 18-fold and 218-fold significantly higher (p < 0.01) levels of transcription in SS than in AS, suggesting a role in gametogenesis-related processes. In contrast, transcription of rom4.4 and 4.5 differed non-significantly between the stages. ROM4 polymorphisms among geographic isolates were essentially restricted to the number of tandem repeats of a 29-amino acid sequence in ROM4.5. This sequence repeat is highly conserved and predicted as antigenic. B. bovis ROMs likely participate in relevant host–pathogen interactions and are possibly useful targets for the development of new control strategies against this pathogen.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherMDPIes_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud públicaes_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud”es_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y laes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourcePathogens 11 (3) : 344 (Marzo 2022)es_AR
dc.subjectTick-borne Diseaseseng
dc.subjectEnfermedades Transmitidas por Garrapatases_AR
dc.subjectBabesiosiseng
dc.subjectBovinaees_AR
dc.subjectBabesia bovises_AR
dc.subjectGene Expressioneng
dc.subjectExpresión Génicaes_AR
dc.subjectPolymorphismeng
dc.subjectPolimorfismoes_AR
dc.subjectBioinformaticseng
dc.subjectBioinformáticaes_AR
dc.subject.otherExperimental Studieseng
dc.subject.otherEstudios Experimentaleses_AR
dc.subject.otherRhomboid Serine Proteaseeng
dc.subject.otherProteasa de Serina Romboidales_AR
dc.titleUnraveling the complexity of the rhomboid serine protease 4 family of Babesia bovis using bioinformatics and experimental studieses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Gallenti, Romina Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentinaes_AR
dc.description.filFil: Gallenti, Romina Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Hussein, Hala E. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidoses_AR
dc.description.filFil: Hussein, Hala E. Cairo University. Faculty of Science. Department of Entomology; Egiptoes_AR
dc.description.filFil: Alzan, Heba F. Washington State University. Department of Veterinary Microbiology and Pathology; Estados Unidoses_AR
dc.description.filFil: Alzan, Heba F. National Research Center. Tick and Tick-Borne Disease Research Unit; Egiptoes_AR
dc.description.filFil: Suarez, Carlos Esteban. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidoses_AR
dc.description.filFil: Suarez, Carlos Esteban. United States Department of Agricultural-Agricultural Research Service. Animal Disease Research Unit; Estados Unidoses_AR
dc.description.filFil: Ueti, Massaro W. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidoses_AR
dc.description.filFil: Ueti, Massaro W. United States Department of Agricultural-Agricultural Research Service. Animal Disease Research Unit; Estados Unidoses_AR
dc.description.filFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Benitez, Daniel Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentinaes_AR
dc.description.filFil: Araujo, Flabio R. EMBRAPA ; Brasiles_AR
dc.description.filFil: Rolls, P. Tick Fever Centre. Department of Agriculture & Fisheries; Australiaes_AR
dc.description.filFil: Sibeko, Kgomotso. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases. Vectors and Vector-borne Diseases Research Programme; Sudáfricaes_AR
dc.description.filFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentinaes_AR
dc.description.filFil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentinaes_AR
dc.description.filFil: Florin-Christensen, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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