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Abstract
Babesia bovis and B. bigemina are tick-transmitted parasites causing bovine babesiosis, characterized by significant morbidity and mortality leading to economic losses to the livestock industry in tropical and subtropical regions worldwide. Animals that recover from acute infection remain carriers with low parasitemia acting as a source of transmission, and often escape detection. An improved diagnosis of a B. bovis and/or B. bigemina infection of carrier [ver mas...]
dc.contributor.authorGanzinelli, Sabrina Belen
dc.contributor.authorByaruhanga, Charles
dc.contributor.authorPrimo, María Evangelina
dc.contributor.authorLukanji, Zinathi
dc.contributor.authorSibeko, Kgomotso
dc.contributor.authorMatjila, Tshepo
dc.contributor.authorNeves, Luis
dc.contributor.authorBenitez, Daniel Francisco
dc.contributor.authorEnkhbaatar, Batmagnai
dc.contributor.authorNugraha, Arifin Budiman
dc.contributor.authorIgarashi, Ikuo
dc.contributor.authorFlorin-Christensen, Mónica
dc.contributor.authorSchnittger, Leonhard
dc.date.accessioned2022-05-10T10:25:01Z
dc.date.available2022-05-10T10:25:01Z
dc.date.issued2022-04
dc.identifier.issn0304-4017
dc.identifier.otherhttps://doi.org/10.1016/j.vetpar.2022.109686
dc.identifier.urihttp://hdl.handle.net/20.500.12123/11840
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0304401722000401
dc.description.abstractBabesia bovis and B. bigemina are tick-transmitted parasites causing bovine babesiosis, characterized by significant morbidity and mortality leading to economic losses to the livestock industry in tropical and subtropical regions worldwide. Animals that recover from acute infection remain carriers with low parasitemia acting as a source of transmission, and often escape detection. An improved diagnosis of a B. bovis and/or B. bigemina infection of carrier animals is enabled by the availability of detection methods with high sensitivity. To this end, two nested PCR assays targeting the cytochrome b (cytb) genes of B. bovis and B. bigemina (cytb-nPCR), have been recently developed and an increased sensitivity with respect to reference protocols has been shown (Romero-Salas et al., 2016). In this study, the specificity against a panel of hemoparasites that potentially co-occur with B. bovis and B. bigemina was demonstrated to ensure applicability of the cytb-nPCR assays in a wide range of regions where bovine babesiosis is endemic. Furthermore, we compared both reported cytb-nPCR assays with reference nPCR and qPCR protocols for (i) their capability to detect carrier animals in the field, and (ii) their reproducibility when performed in different laboratories by independent operators. We show that, in a panel of bovine field samples (n = 100), the cytb-nPCR assays detected a considerably higher number of 25% B. bovis and 61% B. bigemina-positive animals compared to 7% and 20% B. bovis and 55% and 49% B. bigemina-positive animals when tested by reference nPCR and qPCR protocols, respectively. Cytb-nPCRs were also found superior in the detection of carrier animals when field samples from Africa were analyzed. In addition, both the B. bovis and B. bigemina cytb-nPCR assays were independently validated in a single blinded study in three laboratories. Importantly, no significant differences in the number/percentage of infected animals was observed using cytb-nPCR assays. In summary, the cytb-nPCR assays detected a considerably higher number of chronically infected B. bovis and B. bigemina carrier animals compared to reference nPCR and qPCR protocols, when applied in different epidemiological field situations. Furthermore, a high reproducibility between laboratories could be demonstrated.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud públicaes_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E5-I103-001/2019-PD-E5-I103-001/AR./Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud públicaes_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y laes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceVeterinary Parasitology 304 : 109686 (Abril 2022)es_AR
dc.subjectBabesiosises_AR
dc.subjectBovinaees_AR
dc.subjectBabesia bovises_AR
dc.subjectBabesia bigeminaes_AR
dc.subjectCytochrome Beng
dc.subjectCitocromo bes_AR
dc.subjectPCReng
dc.subjectReacción en Cadena de la Polimerasaes_AR
dc.subjectLaboratory Diagnosiseng
dc.subjectDiagnóstico de Laboratorioes_AR
dc.titleInternational interlaboratory validation of a nested PCR for molecular detection of Babesia bovis and Babesia bigemina, causative agents of bovine babesiosises_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Ganzinelli Sabrina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentinaes_AR
dc.description.filFil: Ganzinelli Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Byaruhanga, Charles. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases. Vectors and Vector-borne Diseases Research Programme; Sudáfricaes_AR
dc.description.filFil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentinaes_AR
dc.description.filFil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Lukanji, Zinathi. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases. Vectors and Vector-borne Diseases Research Programme; Sudáfricaes_AR
dc.description.filFil: Sibeko, Kgomotso. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases. Vectors and Vector-borne Diseases Research Programme; Sudáfricaes_AR
dc.description.filFil: Matjila, Tshepo. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases. Vectors and Vector-borne Diseases Research Programme; Sudáfricaes_AR
dc.description.filFil: Neves, Luis. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases. Vectors and Vector-borne Diseases Research Programme; Sudáfricaes_AR
dc.description.filFil: Benitez, Daniel Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentinaes_AR
dc.description.filFil: Enkhbaatar, Batmagnai. Obihiro University of Agriculture and Veterinary Medicine. National Research Center for Protozoan Diseases; Japónes_AR
dc.description.filFil: Enkhbaatar, Batmagnai. Mongolian University of Life Sciences. Institute of Veterinary Medicine. Laboratory of Molecular Genetics; Mongoliaes_AR
dc.description.filFil: Nugraha, Arifin Budiman. Obihiro University of Agriculture and Veterinary Medicine. National Research Center for Protozoan Diseases; Japónes_AR
dc.description.filFil: Nugraha, Arifin Budiman. IPB University. Faculty of Veterinary Medicine. Department of Animal Infectious Diseases and Veterinary Public Health; Indonesiaes_AR
dc.description.filFil: Igarashi, Ikuo. Obihiro University of Agriculture and Veterinary Medicine. National Research Center for Protozoan Diseases; Japónes_AR
dc.description.filFil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentinaes_AR
dc.description.filFil: Florin-Christensen, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentinaes_AR
dc.description.filFil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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