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Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration

Abstract
The ectoparasite Varroa destructor causes serious losses of Apis mellifera colonies and negatively impacts the beekeeping industry around the world. New control methods have been proposed based on the RNA interference technique. Previous reports showed that parasitized honey bees fed with double-stranded RNA (dsRNA) synthesized in vitro reduce the transcription levels of target genes in Varroa mites. An efficient and inexpensive alternative to produce [ver mas...]
The ectoparasite Varroa destructor causes serious losses of Apis mellifera colonies and negatively impacts the beekeeping industry around the world. New control methods have been proposed based on the RNA interference technique. Previous reports showed that parasitized honey bees fed with double-stranded RNA (dsRNA) synthesized in vitro reduce the transcription levels of target genes in Varroa mites. An efficient and inexpensive alternative to produce dsRNA is the use of bacteria capable of achieving high levels of in vivo synthesis. In the present study, dsRNA synthetized in vivo was used to induce gene silencing in V. destructor and evaluate their effect on the survival of both honey bees and the parasitic Varroa mites. The results evidenced that dsRNA fed to the bees engendered gene silencing in mites, inhibiting expression levels of target genes by 50%. Indeed, a reduction of 50% in Varroa survival was observed when bacterially expressed dsRNAs were administered to mite-parasitized bees. Worker bees that were fed with Varroa-targeted dsRNA by oral route showed no survival differences compared to control bees, fed with sucrose or dsRNA-GFP solutions. Our results demonstrated that specific dsRNA over-expressed in bacteria is capable of reducing mite survival by bee-mediated oral administration. This study provides an efficient and low-cost method for dsRNA production to control parasites and honey bee diseases. [Cerrar]
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Author
Muntaabski, Irina;   Scannapieco, Alejandra Carla;   Liendo, María Clara;   Niz, José María;   Russo, Romina Maria;   Salvador, Ricardo;  
Fuente
Journal of Apicultural Research (Published online: 01 Feb 2022)
Date
2022-02
Editorial
Taylor and Francis
ISSN
0021-8839
URI
http://hdl.handle.net/20.500.12123/11611
https://www.tandfonline.com/doi/abs/10.1080/00218839.2022.2028967
DOI
https://doi.org/10.1080/00218839.2022.2028967
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Palabras Claves
Varroa destructor; Honey Bees; Abeja Melífera; Gene Silencing; Silenciamiento Genético; In Vivo Experimentation; Experimentación in Vivo; dsRNA viruses; Synthesis; Síntesis; Apis mellifera;
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