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Abstract
The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, [ver mas...]
dc.contributor.authorLencina, Franco Daniel
dc.contributor.authorLandau, Alejandra Mabel
dc.contributor.authorPrina, Alberto Raul
dc.date.accessioned2022-04-06T10:08:43Z
dc.date.available2022-04-06T10:08:43Z
dc.date.issued2022-02
dc.identifier.issn1422-0067
dc.identifier.otherhttps://doi.org/10.3390/ijms23031814
dc.identifier.urihttp://hdl.handle.net/20.500.12123/11564
dc.identifier.urihttps://www.mdpi.com/1422-0067/23/3/1814
dc.description.abstractThe barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, barley homologs of these genes were identified, and their sequences were analyzed in control and cpm mutant seedlings. A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the Msh1 gene of cpm plants. The relationship between this mutation and the presence of chlorophyll deficiencies was established in progenies from crosses and backcrosses. These results strongly suggest that the mutation identified in the Msh1 gene of the cpm mutant is responsible for the observed plastome instabilities. Interestingly, comparison of mutant phenotypes and molecular changes induced by the barley cpm mutant with those of Arabidopsis MSH1 mutants revealed marked differences.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherMDPIes_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos.es_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuarioes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceInternational Journal of Molecular Sciences 23 (3) : 1814 (Febrero 2022)es_AR
dc.subjectBarleyeng
dc.subjectCebadaes_AR
dc.subjectChloroplastseng
dc.subjectCloroplastoes_AR
dc.subjectMutantseng
dc.subjectMutanteses_AR
dc.subject.otherBarley Chloroplast Mutatoreng
dc.subject.otherMutante del Cloroplasto de Cebadaes_AR
dc.subject.otherMsh1 geneeng
dc.subject.otherGen Msh1es_AR
dc.subject.otherPlastomaes_AR
dc.subject.otherPlastomeeng
dc.titleThe barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 genees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Lencina, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.subtypecientifico


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