Mostrar el registro sencillo del ítem
resumen
Resumen
The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work,
[ver mas...]
dc.contributor.author | Lencina, Franco Daniel | |
dc.contributor.author | Landau, Alejandra Mabel | |
dc.contributor.author | Prina, Alberto Raul | |
dc.date.accessioned | 2022-04-06T10:08:43Z | |
dc.date.available | 2022-04-06T10:08:43Z | |
dc.date.issued | 2022-02 | |
dc.identifier.issn | 1422-0067 | |
dc.identifier.other | https://doi.org/10.3390/ijms23031814 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/11564 | |
dc.identifier.uri | https://www.mdpi.com/1422-0067/23/3/1814 | |
dc.description.abstract | The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, barley homologs of these genes were identified, and their sequences were analyzed in control and cpm mutant seedlings. A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the Msh1 gene of cpm plants. The relationship between this mutation and the presence of chlorophyll deficiencies was established in progenies from crosses and backcrosses. These results strongly suggest that the mutation identified in the Msh1 gene of the cpm mutant is responsible for the observed plastome instabilities. Interestingly, comparison of mutant phenotypes and molecular changes induced by the barley cpm mutant with those of Arabidopsis MSH1 mutants revealed marked differences. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | MDPI | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos. | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | International Journal of Molecular Sciences 23 (3) : 1814 (Febrero 2022) | es_AR |
dc.subject | Barley | eng |
dc.subject | Cebada | es_AR |
dc.subject | Chloroplasts | eng |
dc.subject | Cloroplasto | es_AR |
dc.subject | Mutants | eng |
dc.subject | Mutantes | es_AR |
dc.subject.other | Barley Chloroplast Mutator | eng |
dc.subject.other | Mutante del Cloroplasto de Cebada | es_AR |
dc.subject.other | Msh1 gene | eng |
dc.subject.other | Gen Msh1 | es_AR |
dc.subject.other | Plastoma | es_AR |
dc.subject.other | Plastome | eng |
dc.title | The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Genética | es_AR |
dc.description.fil | Fil: Lencina, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.subtype | cientifico |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
common
-
Artículos científicos [164]