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Abstract
Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the market. In addition, weeds reduce alfalfa yield by about 50 %. Although weeds are the limiting factor for alfalfa production, little progress has been made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This is [ver mas...]
dc.contributor.authorBottero, Ana Emilia
dc.contributor.authorPascuan, Cecilia Gabriela
dc.contributor.authorPagano, Elba Maria
dc.contributor.authorAyub, Nicolás Daniel
dc.contributor.authorSoto, Gabriela Cinthia
dc.contributor.authorJozefkowicz, Cintia
dc.date.accessioned2017-09-06T15:07:09Z
dc.date.available2017-09-06T15:07:09Z
dc.date.issued2016
dc.identifier.issn0721-7714 (Print)
dc.identifier.issn1432-203X (Online)
dc.identifier.otherhttps://doi.org/10.1007/s00299-016-2026-7
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1152
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00299-016-2026-7
dc.description.abstractAlfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the market. In addition, weeds reduce alfalfa yield by about 50 %. Although weeds are the limiting factor for alfalfa production, little progress has been made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This is partially due to the high times and costs needed for the production of vast numbers of transgenic alfalfa events as an empirical approach to bypass the random transgenic silencing and for the identification of an event with optimal transgene expression. In this focus article, we report the complete sequence of pPZP200BAR and the extremely high efficiency of this binary vector in alfalfa transformation, opening the way for rapid and inexpensive production of transgenic events for alfalfa improvement public programs.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourcePlant cell reports 35 (9) : 1987–1990. (September 2016)eng
dc.subjectGenética
dc.subjectGeneticseng
dc.subjectMedicago Sativa
dc.subjectVectores
dc.subjectVectorseng
dc.subjectPlantas Transgénicas
dc.subjectTransgenic Plantseng
dc.subject.otherAlfalfa
dc.titleMinimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAReng
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/acceptedVersioneng
dc.description.origenInst. de Genética "Ewald A. Favret"- IGEAF
dc.gic151653
dc.description.filFil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
dc.description.filFil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
dc.description.filFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.subtypecientifico


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