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resumen

Resumen
Fusarium virguliforme causes the Sudden Death Syndrome, an important disease in soybean crops. In this work, we investigated the defensive response mechanisms in soybean root, at cell wall level, during F. virguliforme infection using an in vitro culture system. We measured total root lignin content by the acetyl bromide method and estimated the in-situ lignin and suberin deposition by confocal microscopy on local and systemic root tissues, i.e. adjacent [ver mas...]
dc.contributor.authorGiachero, María Lorena
dc.contributor.authorMarquez, Nathalie
dc.contributor.authorOrtega, Leandro Ismael
dc.contributor.authorDucasse, Daniel Adrian
dc.date.accessioned2022-03-15T20:22:57Z
dc.date.available2022-03-15T20:22:57Z
dc.date.issued2022-03-07
dc.identifier.otherhttps://doi.org/10.1101/2022.03.07.483270
dc.identifier.urihttp://hdl.handle.net/20.500.12123/11405
dc.identifier.urihttps://www.biorxiv.org/content/10.1101/2022.03.07.483270v1.article-info
dc.description.abstractFusarium virguliforme causes the Sudden Death Syndrome, an important disease in soybean crops. In this work, we investigated the defensive response mechanisms in soybean root, at cell wall level, during F. virguliforme infection using an in vitro culture system. We measured total root lignin content by the acetyl bromide method and estimated the in-situ lignin and suberin deposition by confocal microscopy on local and systemic root tissues, i.e. adjacent and distant to the pathogen entry site respectively. Moreover, the expression dynamics of phenylalanine ammonia lyase (PAL), shikimate/quinate hydroxycinnamoyltransferase (HCT) and cinnamyl alcohol dehydrogenase (CAD) was evaluated by real-time quantitative PCR. The results showed that, although the most significant increment of lignin deposition was observed in the epidermal cells of local tissues, F. virguliforme also induced lignin deposition changes in a sistemic fashion. In fact, inoculated plants presented a higher deposition of lignin in hypodermis and cortex than the control ones, independently of the distance from the inoculum source, while suberin deposition was higher in local zones. Moreover, the gene expression analysis showed an up-regulation of PAL, HCT and CAD genes after the inoculation with the pathogen, which correlates with the cell wall modifications observed in the local tissues. The results presented here suggest that the increase in lignin and suberin deposition during soybean root/F. virguliforme interaction is probably a strategy not only to stop the pathogen entrance, but to provide the plant more time to prepare its defences as well.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherBioRxiv
dc.relationinfo:eu-repograntAgreement/INTA/PNPV-1135024/AR./Defensa en plantas contra fitopatógenos.es_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceBioRxiv : the preprint server for biology. (March 7, 2022)es_AR
dc.subjectLigninseng
dc.subjectBiopolymerseng
dc.subjectCell Wallseng
dc.subjectSojaes_AR
dc.subjectLigninas
dc.subjectSoybeanseng
dc.subjectPared Celular
dc.subjectBiopolímeros
dc.subject.otherSudden Death Syndromeeng
dc.subject.otherSuberineng
dc.titleSoybean responseto initial stage of Fusarium virguliforme infectiones_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/acceptedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Giachero, María Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Ortega, Leandro Ismael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentinaes_AR
dc.description.filFil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.subtypecientifico


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