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Resumen
In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive when applied on the leaves. In this work, we evaluated the survival and localization of Azospirillum brasilense Az39 (Az39) in excised soybean leaves. Scanning electron microscopy and confocal laser [ver mas...]
dc.contributor.authorPuente, Mariana Laura
dc.contributor.authorMaroniche, Guillermo Andrés
dc.contributor.authorPanepucci, Marina Gabriela
dc.contributor.authorSabio Y Garcia, Julia Veronica
dc.contributor.authorGarcía, Julia Elena
dc.contributor.authorCriado, María Victoria
dc.contributor.authorMolina, Romina Micaela
dc.contributor.authorCassán, Fabricio Dario
dc.date.accessioned2021-12-27T16:49:22Z
dc.date.available2021-12-27T16:49:22Z
dc.date.issued2021-05
dc.identifier.issn0266-8254
dc.identifier.otherhttps://doi.org/10.1111/lam.13444
dc.identifier.urihttp://hdl.handle.net/20.500.12123/10996
dc.identifier.urihttps://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/lam.13444
dc.description.abstractIn recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive when applied on the leaves. In this work, we evaluated the survival and localization of Azospirillum brasilense Az39 (Az39) in excised soybean leaves. Scanning electron microscopy and confocal laser scanning microscopy of a red fluorescent-transformed variant of Az39 were used to determine bacterial localization, while the most probable number and plate count methods were applied for bacterial quantification. Microscopic observations indicated a decrease in the number of Az39 cells on the leaf surface at 24 h after treatment, whereas midribs and cell–cell junctions of the inner leaf epidermis became highly populated zones. The presence of Az39 inside xylem vessels was corroborated at 6 h after bacterization. Az39 population did not significantly decrease throughout 24 h. We could visualize Az39 cells on the surface and in internal tissues of soybean leaves and recover them through culture methodologies. These results evidence the survival capacity of Az39 on and inside leaves and suggest a previously unnoticed endophytic potential for this well-known plant growth-promoting rhizobacteria strain.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceLetters in Applied Microbiology 72 (5) : 626-633 (May 2021)es_AR
dc.subjectAzospirillum brasilensees_AR
dc.subjectInoculationeng
dc.subjectSoybeanseng
dc.subjectSoja
dc.subjectInoculación
dc.subject.otherPGPR
dc.titleLocalization and survival of Azospirillum brasilense Az39 in soybean leaveses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Microbiología y Zoología Agrícola (IMYZA)es_AR
dc.description.filFil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Bacterias Promotoras del Crecimiento Vegetal; Argentinaes_AR
dc.description.filFil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Panepucci, Marina Gabriela. Universidad de Buenos Aires. Facultad de Agronomía; Argentinaes_AR
dc.description.filFil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentinaes_AR
dc.description.filFil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentinaes_AR
dc.description.filFil: Criado, Maria Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA)-CONICET; Argentinaes_AR
dc.description.filFil: Molina, Romina Micaela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo. INIAB-CONICET; Argentinaes_AR
dc.description.filFil: Cassán, Fabricio Darío. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo. INIAB-CONICET; Argentinaes_AR
dc.subtypecientifico


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