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resumen

Resumen
In previous works, we have established a correlation between antioxidant system response and tolerance to drought, osmotic stress and photooxidative stress of different wheat cultivars with contrasting drought tolerance. In the present work, a protocol to obtain and transform wheat protoplasts was established. Transgenic protoplasts with Manganese Superoxide Dismutase (Mn-SOD) (E.C.: 1.15.1.1) and Glutathione Reductase (GR) (E.C.: 1.6.4.2) overexpression [ver mas...]
dc.contributor.authorMelchiorre, Mariana
dc.contributor.authorRobert, German
dc.contributor.authorTrippi, Victorio
dc.contributor.authorRacca, Roberto Walter
dc.contributor.authorLascano, Hernan Ramiro
dc.date.accessioned2021-12-23T10:54:41Z
dc.date.available2021-12-23T10:54:41Z
dc.date.issued2008-08-26
dc.identifier.issn0167-6903
dc.identifier.issn1573-5087 (online)
dc.identifier.otherhttps://doi.org/10.1007/s10725-008-9322-3
dc.identifier.urihttp://hdl.handle.net/20.500.12123/10978
dc.identifier.urihttps://link.springer.com/article/10.1007/s10725-008-9322-3
dc.description.abstractIn previous works, we have established a correlation between antioxidant system response and tolerance to drought, osmotic stress and photooxidative stress of different wheat cultivars with contrasting drought tolerance. In the present work, a protocol to obtain and transform wheat protoplasts was established. Transgenic protoplasts with Manganese Superoxide Dismutase (Mn-SOD) (E.C.: 1.15.1.1) and Glutathione Reductase (GR) (E.C.: 1.6.4.2) overexpression in chloroplasts were obtained, and their responses to photooxidative stress were characterized. Protoplasts with Mn-SOD or GR overexpression, showed different responses and tolerance to photooxidative stress. Protoplasts with Mn-SOD overexpression showed lower levels of oxidative damage, higher level of endogenous hydrogen peroxide and a great induction of total SOD and GR activities during photooxidative treatments. In protoplasts with GR overexpression the oxidative damage provoked by the photooxidative treatment was similar to control protoplasts, the GSH content and GSH/GSH + GSSG ratio were higher than control and Mn-SOD transformed protoplast, and total SOD and GR activities were not induced. Our results suggest that the differential responses and tolerance to photooxidative stress given by Mn-SOD or GR overexpression, also depend on the effects of these enzyme activities over the cellular redox state balance, which modulate the responses to photooxidative stress.eng
dc.formatapplication/pdfes_AR
dc.language.isodeues_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourcePlant Growth Regulation 57 : 57-68 (2009)es_AR
dc.subjectGlutathione Reductaseeng
dc.subjectReactive Oxygen Specieseng
dc.subjectSuperoxide Dismutaseeng
dc.subjectGlutatión Reductasaes_AR
dc.subjectSuperóxido Dismutasaes_AR
dc.subjectEspecies de Oxígeno Reactivo
dc.subject.otherPhotooxidative Stresseng
dc.subject.otherWheat Protoplastseng
dc.subject.otherEstrés Fotooxidativoes_AR
dc.titleSuperoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox statees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Fisiología y Recursos Genéticos Vegetaleses_AR
dc.description.filFil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentinaes_AR
dc.description.filFil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentinaes_AR
dc.description.filFil: Trippi, Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentinaes_AR
dc.description.filFil: Trippi, Victorio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentinaes_AR
dc.description.filFil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentinaes_AR
dc.description.filFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentinaes_AR
dc.description.filFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Argentinaes_AR
dc.subtypecientifico


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