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Abstract
Eukaryotes contain three types of lipid kinases that belong to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces cerevisiae, only PI3K class III family members have been identified. These enzymes regulate the innate immune response, intracellular trafficking, autophagy, and senescence. Here, we report that RNAi-mediated downregulation of common bean (Phaseolus vulgaris) PI3K severely impaired symbiosis in composite P. vulgaris [ver mas...]
dc.contributor.authorEstrada-Navarrete, Georgina
dc.contributor.authorCruz-Mireles, Neftaly
dc.contributor.authorLascano, Hernan Ramiro
dc.contributor.authorAlvarado-Affantranger, Xóchitl
dc.contributor.authorHernández-Barrera, Alejandra
dc.contributor.authorBarraza, Aarón
dc.contributor.authorOlivares, Juan E.
dc.contributor.authorArthikala, Manoj-Kumar
dc.contributor.authorCárdenas, Luis
dc.contributor.authorQuinto, Carmen
dc.contributor.authorSanchez, Federico
dc.date.accessioned2021-12-21T10:46:52Z
dc.date.available2021-12-21T10:46:52Z
dc.date.issued2016-09
dc.identifier.issn1040-4651
dc.identifier.issn1532-298X (online)
dc.identifier.otherhttps://doi.org/10.1105/tpc.15.01012
dc.identifier.urihttp://hdl.handle.net/20.500.12123/10954
dc.identifier.urihttps://academic.oup.com/plcell/article/28/9/2326/6098387
dc.description.abstractEukaryotes contain three types of lipid kinases that belong to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces cerevisiae, only PI3K class III family members have been identified. These enzymes regulate the innate immune response, intracellular trafficking, autophagy, and senescence. Here, we report that RNAi-mediated downregulation of common bean (Phaseolus vulgaris) PI3K severely impaired symbiosis in composite P. vulgaris plants with endosymbionts such as Rhizobium tropici and Rhizophagus irregularis. Downregulation of Pv-PI3K was associated with a marked decrease in root hair growth and curling. Additionally, infection thread growth, root-nodule number, and symbiosome formation in root nodule cells were severely affected. Interestingly, root colonization by AM fungi and the formation of arbuscules were also abolished in PI3K loss-of-function plants. Furthermore, the transcript accumulation of genes encoding proteins known to interact with PI3K to form protein complexes involved in autophagy was drastically reduced in these transgenic roots. RNAi-mediated downregulation of one of these genes, Beclin1/Atg6, resulted in a similar phenotype as observed for transgenic roots in which Pv-PI3K had been downregulated. Our findings show that an autophagy-related process is crucial for the mutualistic interactions of P. vulgaris with beneficial microorganismseng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherAmerican Society of Plant Biologistses_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceThe Plant Cell 28 (9) : 2326-2341(September 2016)es_AR
dc.subjectPhaseolus Vulgarises_AR
dc.subjectRhizobiaceaeeng
dc.subjectArbuscular Mycorrhizaeng
dc.subjectMicorrizas Arbusculares
dc.subjectQuinasas
dc.subjectKinaseseng
dc.subject.otherPoroto
dc.titleAn Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungies_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Fisiología y Recursos Genéticos Vegetaleses_AR
dc.description.filFil: Estrada-Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; Méxicoes_AR
dc.description.filFil: Cruz-Mireles, Neftaly. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; Méxicoes_AR
dc.description.filFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentinaes_AR
dc.description.filFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentinaes_AR
dc.description.filFil: Alvarado-Affantranger, Xóchitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Laboratorio Nacional de Microscopía Avanzada; Méxicoes_AR
dc.description.filFil: Hernández-Barrera, Alejandra. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; Méxicoes_AR
dc.description.filFil: Barraza, Aarón. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; Méxicoes_AR
dc.description.filFil: Olivares, Juan E. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; Méxicoes_AR
dc.description.filFil: Arthikala, Manoj-Kumar. Universidad Nacional Autónoma de México. Escuela Nacional de Estudios Superiores-Unidad León; Méxicoes_AR
dc.description.filFil: Cárdenas, Luis. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; Méxicoes_AR
dc.description.filFil: Quinto, Carmen. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; Méxicoes_AR
dc.description.filFil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; Méxicoes_AR
dc.subtypecientifico


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