resumen
Abstract
Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found
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dc.contributor.author | Saraullo, Vanina Rosa | |
dc.contributor.author | Grune Loffler, Sylvia | |
dc.contributor.author | Florin-Christensen, Mónica | |
dc.contributor.author | Watanabe, Olivia | |
dc.contributor.author | Hamer, Micaela | |
dc.contributor.author | Martinez, Mara Leila | |
dc.contributor.author | Brihuega, Bibiana Felicitas | |
dc.date.accessioned | 2021-11-15T13:31:05Z | |
dc.date.available | 2021-11-15T13:31:05Z | |
dc.date.issued | 2021-10 | |
dc.identifier.issn | 0147-9571 | |
dc.identifier.other | https://doi.org/10.1016/j.cimid.2021.101689 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/10776 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/abs/pii/S0147957121000813 | |
dc.description.abstract | Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found with intermediate or non-pathogenic species, or with other microorganisms, demostrating its high analytical specificity. The estimated analytical sensitivity was higher in serum samples than in blood or urine samples (6−9 × 102 lept/mL and 6−9 × 105 and 6−9 × 106 lept/mL, respectively). Multiple sequence alignment of the target region from different pathogenic Leptospira species confirmed that this gene region is highly conserved among these species, with few single nucleotide polymorphisms. The ligb-ct PCR here developed appears as a useful tool for the molecular diagnosis of leptospirosis. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Elsevier | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Comparative Immunology, Microbiology and Infectious Diseases 78 : 101689 (October 2021) | es_AR |
dc.subject | Leptospirosis | es_AR |
dc.subject | Enfermedades de los Animales | es_AR |
dc.subject | Animal Diseases | eng |
dc.subject | PCR | es_AR |
dc.subject | Diagnóstico | es_AR |
dc.subject | Diagnosis | eng |
dc.subject | Genética | es_AR |
dc.subject | Genetics | eng |
dc.subject | Leptospira | |
dc.title | Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | Instituto de Patobiología | es_AR |
dc.description.fil | Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. | es_AR |
dc.description.fil | Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. | es_AR |
dc.description.fil | Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Jacobsen, Mónica Ofelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. | es_AR |
dc.description.fil | Fil: Jacobsen, Mónica Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. | es_AR |
dc.description.fil | Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. | es_AR |
dc.description.fil | Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. | es_AR |
dc.description.fil | Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. | es_AR |
dc.description.fil | Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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