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Since their discovery, plant-growth promoting rhizobacteria from the genus Azospirillum have been sub-jected to intensive research due to their biotechnological potential as crop inoculants. Phylogeneticanalysis of Azospirillum spp. is carried out by 16S rRNA sequencing almost exclusively, but inconsis-tencies and low confidence often arise when working with close species. In this work, it was observedthat these difficulties might be explained by a high
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dc.contributor.author | Maroniche, Guillermo Andrés | |
dc.contributor.author | Garcia, Julia Elena | |
dc.contributor.author | Salcedo, Florencia | |
dc.contributor.author | Creus, Cecilia Mónica | |
dc.date.accessioned | 2017-08-24T13:50:00Z | |
dc.date.available | 2017-08-24T13:50:00Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0944-5013 | |
dc.identifier.other | https://doi.org/10.1016/j.micres.2016.11.009 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/1032 | |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0944501316307327?via%3Dihub | |
dc.description.abstract | Since their discovery, plant-growth promoting rhizobacteria from the genus Azospirillum have been sub-jected to intensive research due to their biotechnological potential as crop inoculants. Phylogeneticanalysis of Azospirillum spp. is carried out by 16S rRNA sequencing almost exclusively, but inconsis-tencies and low confidence often arise when working with close species. In this work, it was observedthat these difficulties might be explained by a high number of rRNA operons with considerable inter-genic variability within Azospirillum genomes. To search for alternative genetic markers from a list ofhousekeeping genes, the correlation between pairwise gene and whole-genome similarities was exam-ined. Due to its good performance, rpoD was selected for further analyses. Genus-specific primers for thePCR-amplification and sequencing of rpoD from Azospirillum spp. were designed and tested on 16 typestrains of different species. The sequences obtained were used for inferring a phylogenetic tree of thegenus, which was in turn used as a reference to successfully identify a collection of 31 azospirilla iso-lated from many different locations of Argentine. In addition, several strains that might represent novelspecies were detected. The results indicate that the sequencing of rpoD is a suitable alternative methodfor a confident molecular identification in Azospirillum spp. | |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.rights | info:eu-repo/semantics/openAccess | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Microbiological research 195 : 1-10. (January 2017) | eng |
dc.subject | Azospirillum | |
dc.subject | Marcadores Genéticos | |
dc.subject | Genetic Markers | eng |
dc.subject | Rhizobacteria | eng |
dc.subject | Rizobacterias | |
dc.subject | Genotypes | eng |
dc.subject | Genotipos | |
dc.title | Molecular identification of Azospirillum spp.: limitations of 16S rRNA and qualities of rpoD as genetic markers | eng |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/acceptedVersion | eng |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Inst. de Microbiología y Zoología Agrícola IMyZA | |
dc.gic | 152964 | |
dc.description.fil | Fil: Maroniche, Guillermo Andrés. Unidad Integrada Balcarce-Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias-Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina | |
dc.description.fil | Fil: Salcedo, Florencia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina | |
dc.subtype | cientifico |
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