Show simple item record

resumen

Abstract
Low arbuscular-mycorrhizal (AM) sporulation in arid field soils limits our knowledge of indigenous species when diversity studies are based only on spore morphology. Our aim was to use different approaches (i.e., spore morphological approach and PCR–SSCP (single-strand-conformation-polymorphism) analysis after trap plant multiplication strategies to improve the knowledge of the current richness of glomalean AM fungi (Glomerales; Glomeromycota) from the [ver mas...]
dc.contributor.authorCovacevich, Fernanda
dc.contributor.authorHernandez Guijarro, Keren
dc.contributor.authorCrespo, Esteban
dc.contributor.authorLumini, Erica
dc.contributor.authorRivero Mega, María Soledad
dc.contributor.authorLugo, Mónica
dc.date.accessioned2021-09-14T11:45:50Z
dc.date.available2021-09-14T11:45:50Z
dc.date.issued2021-08-30
dc.identifier.issn2223-7747
dc.identifier.otherhttps://doi.org/10.3390/plants10091803
dc.identifier.urihttp://hdl.handle.net/20.500.12123/10249
dc.identifier.urihttps://www.mdpi.com/2223-7747/10/9/1803/html
dc.description.abstractLow arbuscular-mycorrhizal (AM) sporulation in arid field soils limits our knowledge of indigenous species when diversity studies are based only on spore morphology. Our aim was to use different approaches (i.e., spore morphological approach and PCR–SSCP (single-strand-conformation-polymorphism) analysis after trap plant multiplication strategies to improve the knowledge of the current richness of glomalean AM fungi (Glomerales; Glomeromycota) from the Argentine Puna. Indigenous propagules from two pristine sites at 3870 and 3370 m of elevation were multiplied using different host plants; propagation periods (2–6 months), and subculture cycles (1; 2; or 3) from 5 to 13 months. The propagule multiplication experiment allowed the detection of different glomoid taxa of Funneliformis spp. and Rhizoglomus spp., which were considered cryptic species since they had never been found in Puna soils before. On the other hand; almost all the generalist species previously described were recovered from cultures; except for Glomus ambisporum. Both plant host selection and culture times are critical for Glomerales multiplication. The SSCP analysis complemented the morphological approach and showed a high variability of Glomus at each site; revealing the presence of Funneliformis mosseae. This study demonstrates that AMF trap culture (TC) is a useful strategy for improving the analysis of AM fungal diversity/richness in the Argentinean highlands.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)es_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E2-I037-002/2019-PD-E2-I037-002/AR./Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso sueloes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourcePlants 10 (9) : 1803 (September 2021)es_AR
dc.subjectSueloes_AR
dc.subjectSoileng
dc.subjectHongoses_AR
dc.subjectFungieng
dc.subjectBiodiversidades_AR
dc.subjectBiodiversityeng
dc.subjectMicorrizas Arbusculareses_AR
dc.subjectArbuscular Mycorrhizaeng
dc.subjectPropáguloses_AR
dc.subjectSetseng
dc.subjectZona de Montañaes_AR
dc.subjectHighlandseng
dc.subjectArgentinaes_AR
dc.titleArbuscular mycorrhizal fungi from argentinean highland puna soils unveiled by propagule multiplicationes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Covacevich, Fernanada. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Investigación Biológicas Aplicadas. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina.es_AR
dc.description.filFil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.es_AR
dc.description.filFil: Crespo, Esteban. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.es_AR
dc.description.filFil: Lumini, Erica. Institute for Sustainable Plant Protection of National Research Council; Italia.es_AR
dc.description.filFil: Rivero Mega, María Soledad. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.es_AR
dc.description.filFil: Lugo, Mónica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina.es_AR
dc.description.filFil: Lugo, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis; Argentina. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina.es_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record