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Browsing by Author "20123105479"
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A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences
Iraola, Gregorio; Pérez, Ruben; Betancor, Laura; Marandino, Ana; Morsella, Claudia Graciela; Mendez, Maria Alejandra; Paolicchi, Fernando; Piccirillo, Alessandra; Tomás, Gonzalo; Velilla, Alejandra Vanesa; Calleros, Lucía (BMC, 2016-12)Background: Campylobacter fetus is a pathogen of major concern for animal and human health. The species shows a great intraspecific variation, with three subspecies: C. fetus subsp. fetus, C. fetus subsp. venerealis, and ... -
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
Delpiazzo, Rafael; Barcellos, Maila; Barros, Sofia; Bentacor, Laura; Fraga, Martin; Gil, Jorge; Iraola, Gregorio; Morsella, Claudia Graciela; Paolicchi, Fernando; Perez, Ruben; Riet Correa, Franklin; Sanguinetti, Margarita; Silva Silveira, Caroline da; Caballeros, Lucía (Elsevier, 2020-12-24)Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders ... -
Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle
Garbaccio, Sergio Gabriel; Macias, Analía; Shimizu, Ernesto; Paolicchi, Fernando; Pezzone, Natalia; Zapata, Laura; Abdala, Alejandro Ariel; Tarabla, Hector Dante; Peyru, Maite; Caimi, Karina Cynthia; Zumarraga, Martin Jose; Canal, Ana María; Cataldi, Angel Adrian; Magnano, Gabriel (Taylor & Francis, 2014-01)Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects approximately 5% of Argentine cattle. The aim of this research was to study if it is possible to infer the degree of virulence of ... -
BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology
Eirin, Maria Emilia; Carignano, Hugo Adrian; Shimizu, Ernesto; Pando, Maria de los Angeles; Zumarraga, Martin Jose; Magnano, Gabriel; Macías, Analía; Garbaccio, Sergio Gabriel; Huertas, Pablo Sebastian; Morsella, Claudia Graciela; Ferrara Muñiz, Ximena; Cataldi, Angel Adrian; Paolicchi, Fernando; Poli, Mario Andres (Elsevier, 2020-06)Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and disseminated worldwide. In Argentina, the highest prevalence occurs in dairy areas. BoLA DRB3.2 is related to the adaptive immunity in mycobacterial infections. ... -
Brote de abortos por Brucella suis en granja porcina de Buenos Aires (Argentina)
Micheloud, Juan Francisco; Perez, M.; Margineda, Carlos Augusto; Salomon Buffa, M.; Morell, Eleonora Lidia; Paolicchi, Fernando; Fiorentino, María Andrea; Campero, Carlos Manuel (2012)La brucelosis por Brucella suis es una enfermedad infectocontagiosa que produce grandes pérdidas económicas en la industria porcina y tiene gran impacto sobre la salud pública por tratarse de una zoonosis. Aquí se describe ... -
Campylobacter fetus releases S-layered and immunoreactive outer membrane vesicles = Campylobacter fetus secreta vesículas de membrana externa inmunorreactivas que conservan la capa S
Farace, Pablo; Cravero, Silvio Lorenzo Pedro; Taibo, Catalina Beatriz; Diodati, Julian Angel; Morsella, Claudia Graciela; Paolicchi, Fernando; Sabio Y Garcia, Julia Veronica; Gioffre, Andrea (Elsevier, 2021-08)The study of outer membrane vesicles (OMVs) became relevant because of their probable important role in the transfer of virulence factors to host cells. Campylobacter fetus is mainly a mammal pathogen whose virulence ... -
Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
Manes, Jorgelina; Fiorentino, María Andrea; Kaiser, German Gustavo; Hozbor, Federico Andres; Alberio, Ricardo; Sánchez, Esteban O.; Paolicchi, Fernando (Elsevier, 2010-11)The objective of this study was to characterize the vaginal bacterial flora and subsequent pregnancy rates after AI in sheep subjected to 4 different estrous synchronization regimes – the use of two intravaginal devices ... -
Corynebacterium pseudotuberculosis biovar ovis: evaluación de la sensibilidad antibiótica in vitro
Gallardo, Adriana Alicia; Toledo, Rocío Andrea; Gonzalez Pasayo, Ramón Alejandro; de Carvalho Azevedo, Vasco Ariston; Robles, Carlos Alejandro; Paolicchi, Fernando; Estevao Belchior, Silvia Graciela (Asociación Argentina de Microbiología, 2019-12)Los objetivos de este trabajo fueron estudiar la sensibilidad antibiótica de aislamientos de Corynebacterium pseudotuberculosis procedentes de pequeños rumiantes e investigar la presencia de integrones que contienen genes ... -
Current trends in bovine abortion in Argentina
Morrell, Eleonora Lidia; Campero, Carlos Manuel; Canton, German Jose; Odeon, Anselmo Carlos; Moore, Prando Dadin; Odriozola, Ernesto Raul; Paolicchi, Fernando; Fiorentino, María Andrea (Colegio Brasileiro de Pathología Animal, 2019-01)Bovine abortion is an important cause of significant economic losses in beef and dairy herds. This syndrome is usually difficult to diagnose. The aim of this study was to characterize bovine abortion causes in Argentina ... -
Detección de serovares de Salmonella en terneros de crianza artificial de la región lechera Mar y Sierras, Argentina
Bilbao, Gladys; Malena, Rosana Claudia; Passucci, Juan; Pinto de Almeida Castro, Aldana; Paolicchi, Fernando; Soto, Pedro; Cantón, Juliana; Monteavaro, Cristina (Elsevier, 2019-01-09)La salmonelosis es una de las enfermedades bacterianas que afectan el tracto digestivo de los terneros y provocan en ellos diarrea. Con el objetivo de estudiar la prevalencia de los distintos serovares de Salmonella en ... -
Eficiencia del cultivo bacteriológico y de la inmunofluorescencia en la detección de Campylobacter fetus en fluidos genitales bovinos = Efficiency of bacteriological culture and the immunofluorescent assay to detect Campylobacter fetus in bovine genital fluids
Marcellino, Romanela; Morsella, Claudia Graciela; Cano, Dora Beatriz; Paolicchi, Fernando (Asociación Argentina de Microbiología, 2015-07)La campilobacteriosis genital bovina es una enfermedad reproductiva que afecta la producción bovina. Es causada por las subespecies de Campylobacter fetus, C. fetus fetus (Cff) y C. fetus venerealis (Cfv). El objetivo de ... -
Identificación de factores de riesgo de tuberculosis bovina en rodeos lecheros
Garro, Carlos Javier (Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, 2009-09)La tuberculosis bovina (TBB), producida por el Mycobacterium bovis (M. bovis), es una enfermedad zoonótica endémica en los rodeos bovinos de la Argentina. La información sobre la asociación entre el manejo y la ocurrencia ... -
Identificación y caracterización bacteriológica y molecular de cepas de Mycobacterium avium subsp. paratuberculosis en productos lácteos y agua.
Cirone, Karina Mariela (Universidad Nacional del Centro de la Provincia de Buenos Aires, Facultad de Ciencias Veterinarias, Tandil, Argentina, 2015)La paratuberculosis (PTBC) o enfermedad de Johne es una enteritis crónica producida por Mycobacterium avium subsp. paratuberculosis (MAP) que afecta a bovinos y otras especies. MAP ha sido vinculado en humanos a una ... -
Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis
Eirin, Maria Emilia; Macías, Analía; Magnano, Gabriel; Morsella, Claudia Graciela; Mendez, Laura Luján; Blanco, Federico Carlos; Bianco, María Veronica; Severina, Walter; Alito, Alicia Elsa; Pando, Maria de los Angeles; Singh, Mahavir G.; Spallek, Ralph; Paolicchi, Fernando; Bigi, Fabiana; Cataldi, Angel Adrian (Elsevier, 2015-12)Bovine tuberculosis (bTB) is a common zoonotic disease, caused by Mycobacterium bovis (M. bovis), responsible for significant economic losses worldwide. Its diagnosis is based on the detection of cell mediated immunity ... -
Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models
Colavecchia, Silvia Beatriz; Fernández, Bárbara; Jolly, Ana; Minatel, Leonardo; Hajos, Silvia Elvira; Paolicchi, Fernando; Mundo, Silvia Leonor (Elsevier, 2016-08)Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of ruminant paratuberculosis. The aim of this study was to evaluate the biological behavior of different Argentinean strains of MAP in two bovine ... -
Isolation of Campylobacter fetus subsp. venerealis from seminal vesicle of a naturally challenged bull
García, Juan Agustín; Gioffre, Andrea; Acuña, Juaquín; Mendez, Maria Alejandra; Morsella, Claudia Graciela; Aller Atucha, Juan Florencio; Paolicchi, Fernando (Springer Verlag, 2021-09-03)Campylobacter fetus is a well-recognized pathogen that affects reproductive rate in cattle. In the present study, two Angus bulls were kept (39 days) separately with a group of heifers experimentally infected withCampylobacter ... -
L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus
Farace, Pablo Daniel; Morsella, Claudia Graciela; Cravero, Silvio Lorenzo Pedro; Sioya, Bernardo Arturo; Amadio, Ariel; Paolicchi, Fernando; Gioffre, Andrea (2019-11)Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive ... -
Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of Brucella abortus-bovine infected fetuses
Fiorentino, María Andrea; Campero, Carlos Manuel; Paolicchi, Fernando; Barbeito, Claudio Gustavo (2018)Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or ... -
Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates
Encinas, Micaela; Marfil, Maria Jimena; Garbaccio, Sergio Gabriel; Barandiaran, Soledad; Huertas, Pablo Sebastian; Morsella, Claudia Graciela; Macias, Analía; Magnano, Gabriel; Zapata, L.; Bigi, Fabiana; Cataldi, Angel Adrian; Paolicchi, Fernando; Zumarraga, Martin Jose; Eirin, Maria Emilia (Elsevier, 2018-07)ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, ... -
Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR
Gilardoni, Liliana Rosa; Fernández, Bárbara; Morsella, Claudia Graciela; Mendez, Laura Luján; Jar, Ana María; Paolicchi, Fernando; Mundo, Silvia Leonor (Elsevier, 2016-06)The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic ...