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Enteric viruses are pathogens associated with foodand waterborne outbreaks. The recovery of viruses from food or water samples is affected by the procedures applied to detect and concentrate them. The incorporation of an internal process control virus to the analyses allows monitoring the performance of the methodology. The aim of this study was to produce a recombinant adenovirus (rAdV) and apply it together with bacteriophage PP7 as process controls.
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dc.contributor.author | Blanco Fernández, María Dolores | |
dc.contributor.author | Barrios, Melina Elizabeth | |
dc.contributor.author | Cammarata, Robertina Viviana | |
dc.contributor.author | Torres, Carolina | |
dc.contributor.author | Taboga, Oscar Alberto | |
dc.contributor.author | Mbayed, Viviana Andrea | |
dc.date.accessioned | 2017-08-11T11:50:23Z | |
dc.date.available | 2017-08-11T11:50:23Z | |
dc.date.issued | 2017-05 | |
dc.identifier.issn | 1432-0614 (Online) | |
dc.identifier.issn | 0175-7598 (Print) | |
dc.identifier.other | https://doi.org/10.1007/s00253-017-8244-2 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/951 | |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs00253-017-8244-2 | |
dc.description.abstract | Enteric viruses are pathogens associated with foodand waterborne outbreaks. The recovery of viruses from food or water samples is affected by the procedures applied to detect and concentrate them. The incorporation of an internal process control virus to the analyses allows monitoring the performance of the methodology. The aim of this study was to produce a recombinant adenovirus (rAdV) and apply it together with bacteriophage PP7 as process controls. The rAdV carries a DNA construction in its genome to differentiate it from wild-type adenovirus by qPCR. The stability of both control viruses was evaluated at different pH conditions. The rAdV was stable at pH 3, 7, and 10 for 18 h. PP7 infectious particles were stable at pH 7 and showed a 2.14 log reduction at pH 10 and total decay at pH 3 after 18 h. Three virus concentration methods were evaluated: hollow-fiber tap water ultrafiltration, wastewater ultracentrifugation, and elution-PEG precipitation from lettuce. Total and infectious viruses were quantified and their recoveries were calculated. Virus recovery for rAdV and PP7 by ultrafiltration showed a wide range (2.10–84.42 and 13.54–84.62%, respectively), whereas that by ultracentrifugation was 5.05–13.71 and 6.98–13.27%, respectively. The performance of ultracentrifugation to concentrate norovirus and enteroviruses present in sewage was not significantly different to the recovery of control viruses. For detection of viruses from lettuce, genomic copies of PP7 were significantly more highly recovered than adenovirus (14.74–18.82 and 0.00–3.44%, respectively). The recovery of infectious virus particles was significantly affected during sewage ultracentrifugation and concentration from lettuce. The simultaneous use of virus controls with dissimilar characteristics and behaviors might resemble different enteric viruses. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Applied microbiology and biotechnology 101 (10) : 4289–4298. (2017) | eng |
dc.subject | Food Technology | eng |
dc.subject | Tecnología de Alimentos | |
dc.subject | Wterborne Diseases | eng |
dc.subject | Enfermedades Transmitidas por Agua | |
dc.subject | Control de Enfermedades | |
dc.subject | Disease Control | eng |
dc.title | Comparison of internal process control viruses for detection of food and waterborne viruses | eng |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.description.origen | Inst. de Biotecnología | |
dc.gic | 154160 | |
dc.description.fil | Fil: Blanco Fernández, María Dolores. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Barrios, Melina Elizabeth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Virología; Argentina. Ministerio de Ciencia y Tecnología. Agencia Nacional de Promoción Científica y Tecnológica; Argentina | |
dc.description.fil | Fil: Cammarata, Robertina Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Torres, Carolina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Mbayed, Viviana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.subtype | cientifico |
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