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Abstract
Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy [ver mas...]
dc.contributor.authorSalimena, Alessandra P.S.
dc.contributor.authorLange, Carla C.
dc.contributor.authorCamussone, Cecilia
dc.contributor.authorSignorini, Marcelo
dc.contributor.authorCalvinho, Luis Fernando
dc.contributor.authorBrito, María A.V.P.
dc.contributor.authorBorges, Cristiano A.V.
dc.contributor.authorGuimaraes, Alessandro S.
dc.contributor.authorRibeiro, Joao B.
dc.contributor.authorMendonca, Leticia C.
dc.contributor.authorPiccoli, Roberta H.
dc.coverage.spatialBrazil (nation)
dc.date.accessioned2017-08-07T13:26:11Z
dc.date.available2017-08-07T13:26:11Z
dc.date.issued2016-12
dc.identifier.issn1573-7446
dc.identifier.otherhttps://doi.org/10.1007/s11259-016-9658-5
dc.identifier.urihttp://hdl.handle.net/20.500.12123/910
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs11259-016-9658-5
dc.description.abstractStaphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.eng
dc.formatapplication/pdf
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourceVeterinary research communications 40 (3-4) : 97-106. (2016)
dc.subjectStaphylococcus aureus
dc.subjectGanado de Leche
dc.subjectDairy Cattleeng
dc.subjectGenotipos
dc.subjectGenotypeseng
dc.subjectFenotipos
dc.subjectPhenotypeseng
dc.subjectLeche
dc.subjectMilkeng
dc.titleGenotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.description.origenEEA Rafaela
dc.gic150525
dc.description.filFil: Salimena, Alessandra P.S. EMBRAPA Dairy Cattle Research Center; Brasil. Federal University of Lavras. Food Science Department; Brasil
dc.description.filFil: Lange, Carla C. EMBRAPA Dairy Cattle Research Center; Brasil
dc.description.filFil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.filFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.filFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.filFil: Brito, María A.V.P. EMBRAPA Dairy Cattle Research Center; Brasil
dc.description.filFil: Borges, Cristiano A.V. EMBRAPA Dairy Cattle Research Center; Brasil
dc.description.filFil: Guimaraes, Alessandro S. EMBRAPA Dairy Cattle Research Center; Brasil
dc.description.filFil: Ribeiro, Joao B. EMBRAPA Dairy Cattle Research Center; Brasil
dc.description.filFil: Mendonca, Leticia C. EMBRAPA Dairy Cattle Research Center; Brasil
dc.description.filFil: Piccoli, Roberta H. Federal University of Lavras. Food Science Department; Brasil
dc.subtypecientifico


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