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Abstract
Neospora caninum is a protozoan parasite that causes abortion and reproductive failure in small ruminants. We validated and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein (tSAG1) from N. caninum for the detection of anti-N. caninum antibodies in sheep and goat flocks. The assay was validated using 80 positive and 142 negative serum samples from sheep and goats analyzed by IFAT and immunoblot (IB). [ver mas...]
dc.contributor.authorNovoa, María Belen
dc.contributor.authorAguirre, Nerina
dc.contributor.authorOrmaechea, Nadia
dc.contributor.authorPalmero, Sebastián
dc.contributor.authorRouzic, Lisandro
dc.contributor.authorValentini, Beatriz Susana
dc.contributor.authorSarli, Macarena
dc.contributor.authorOrcellet, Viviana Mercedes
dc.contributor.authorMarengo, Rafael
dc.contributor.authorVanzini, Victor Rene
dc.contributor.authorPrimo, María Evangelina
dc.dateinfo:eu-repo/date/embargoEnd/2021-08-11
dc.date.accessioned2020-08-11T11:54:42Z
dc.date.available2020-08-11T11:54:42Z
dc.date.issued2020-08
dc.identifier.issn0304-4017
dc.identifier.otherhttps://doi.org/10.1016/j.vetpar.2020.109201
dc.identifier.urihttp://hdl.handle.net/20.500.12123/7699
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0304401720301813
dc.description.abstractNeospora caninum is a protozoan parasite that causes abortion and reproductive failure in small ruminants. We validated and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein (tSAG1) from N. caninum for the detection of anti-N. caninum antibodies in sheep and goat flocks. The assay was validated using 80 positive and 142 negative serum samples from sheep and goats analyzed by IFAT and immunoblot (IB). ciELISAtSAG1 was then used to evaluate the prevalence of anti-N. caninum antibodies in 1449 goats from 143 flocks and 385 sheep from 40 flocks and compared to IFAT. The prevalence of anti-Toxoplasma gondii antibodies was evaluated by IFAT. The ciELISAtSAG1 cut-off was ≥ 36 percent inhibition, with a diagnostic sensitivity of 100.0% (95% CI = 95.4–100.0%) and a diagnostic specificity of 98.6% (95% CI = 95.0–99.8%) relative to the agreement between IFAT and IB. The field evaluation revealed a concordance between ciELISAtSAG1 and IFAT of 97.4%, with an agreement (κ) of 0.90 for sheep sera, and a concordance of 96.5% with κ = 0.85 for goat sera. The overall prevalence of anti-N. caninum antibodies in sheep was 14.3% by IFAT and 15.8% by ciELISAtSAG1. In goats, prevalence was 12.9% by IFAT and 14.6% by ciELISAtSAG1. The overall prevalence of anti-T. gondii antibodies was 28.8% in goats and 43.8% in sheep. The ciELISAtSAG1 could be useful for large-scale detection of anti-N. caninum antibodies in sheep and goats, and for seroepidemiological investigations due to its appropriate sensitivity and specificity, and the simplicity of production.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_AR
dc.sourceVeterinary Parasitology : 109201 (Available online 7 August 2020)es_AR
dc.subjectOvinoses_AR
dc.subjectSheepeng
dc.subjectCaprinoses_AR
dc.subjectGoatseng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectNeospora caninumes_AR
dc.subjectDiagnósticoes_AR
dc.subjectDiagnosiseng
dc.subjectELISAes_AR
dc.subjectToxoplasma gondiies_AR
dc.subjectAnticuerposes_AR
dc.subjectAntibodieseng
dc.titleValidation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goatses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/acceptedVersiones_AR
dc.description.origenEEA Rafaelaes_AR
dc.description.filFil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Aguirre, Nerina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina.es_AR
dc.description.filFil: Ormaechea, Nadia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Palmero, Sebastián. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Rouzic, Lisandro. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Orcellet, Viviana Mercedes. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Marengo, Rafael. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Vanzini Victor Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.subtypecientifico


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