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Resumen
Shiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food that may cause severe illness in human beings. Thus, systems for STEC detection in food should have increasingly higher sensitivity and specificity. Here we compared six commercial systems for non-O157 STEC detection in meat and vegetables and determined their sensitivity, specificity and repeatability. A total of 46 samples (meat n = 23; chard n = 23) were
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dc.contributor.author | Costa, Magdalena | |
dc.contributor.author | Sucari, Adriana | |
dc.contributor.author | Epszteyn, Sergio | |
dc.contributor.author | Oteiza, Juan Martín | |
dc.contributor.author | Gentiluomo, Jimena | |
dc.contributor.author | Melamed, Celia | |
dc.contributor.author | Figueroa, Yamila | |
dc.contributor.author | Mingorance, Santiago Emmanuel | |
dc.contributor.author | Grisaro, Agustina | |
dc.contributor.author | Spioussas, Silvia | |
dc.contributor.author | Buffoni Almeida, Mariana | |
dc.contributor.author | Caruso, Mailen | |
dc.contributor.author | Pontoni, Andrés | |
dc.contributor.author | Signorini, Marcelo | |
dc.contributor.author | Leotta, Gerardo Anibal | |
dc.date.accessioned | 2019-08-26T11:55:22Z | |
dc.date.available | 2019-08-26T11:55:22Z | |
dc.date.issued | 2019-12 | |
dc.identifier.issn | 0740-0020 | |
dc.identifier.issn | 1095-9998 | |
dc.identifier.other | https://doi.org/10.1016/j.fm.2019.103273 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0740002019304332 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/5684 | |
dc.description.abstract | Shiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food that may cause severe illness in human beings. Thus, systems for STEC detection in food should have increasingly higher sensitivity and specificity. Here we compared six commercial systems for non-O157 STEC detection in meat and vegetables and determined their sensitivity, specificity and repeatability. A total of 46 samples (meat n = 23; chard n = 23) were experimentally contaminated with strains O26:H11, O45:H-, O103:H2, O111:NM, O121:H19 and O145:NM isolated in Argentina. Strain detection was confirmed by isolation according to ISO 13136:2012. Detection of the stx and eae genes in meat samples was highly satisfactory with all commercial kits, but only five had 100% sensitivity and specificity in chard. Of four kits evaluated for serogroup detection, three had 100% sensitivity and specificity, and one had 93.7% sensitivity and 100% specificity. All kits were adequate to analyze meat but not vegetable samples, and were not therefore validated for the latter matrix. The challenge for microbiology laboratories is to identify the advantages and disadvantages of the available kits for STEC detection in food based on a clear knowledge of the particular needs of each laboratory. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Elsevier | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Food Microbiology 84 : 103273 (December 2019) | es_AR |
dc.subject | Escherichia coli | es_AR |
dc.subject | Toxinas | es_AR |
dc.subject | Toxins | eng |
dc.subject | Carne | es_AR |
dc.subject | Meat | eng |
dc.subject | Hortalizas | es_AR |
dc.subject | Vegetables | eng |
dc.subject | Identificación | es_AR |
dc.subject | Identification | eng |
dc.subject | Control de Enfermedades | es_AR |
dc.subject | Disease Control | eng |
dc.subject.other | Shiga toxin | es_AR |
dc.title | Comparison of six commercial systems for the detection of non-O157 STEC in meat and vegetables | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | EEA Rafaela | es_AR |
dc.description.fil | Fil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina | es_AR |
dc.description.fil | Fil: Sucari, Adriana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina | es_AR |
dc.description.fil | Fil: Epszteyn, Sergio. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina | es_AR |
dc.description.fil | Fil: Oteiza, Juan Martín. Centro de Investigación y Asistencia Técnica a la Industria. Laboratorio de Microbiología de los Alimentos (Neuquén); Argentina | es_AR |
dc.description.fil | Fil: Gentiluomo, Jimena. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina | es_AR |
dc.description.fil | Fil: Melamed, Celia. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina | es_AR |
dc.description.fil | Fil: Figueroa, Yamila. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina | es_AR |
dc.description.fil | Fil: Mingorance, Santiago Emmanuel. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina | es_AR |
dc.description.fil | Fil: Grisaro, Agustina. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina | es_AR |
dc.description.fil | Fil: Spioussas, Silvia. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina | es_AR |
dc.description.fil | Fil: Buffoni Almeida, Mariana. Centro Estudios Infectológicos “Dr. Daniel Stamboulian”. División Higiene y Seguridad Alimentaria y Ambiental; Argentina | es_AR |
dc.description.fil | Fil: Caruso, Mailen. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina | es_AR |
dc.description.fil | Fil: Pontoni, Andrés. Buenos Aires. Dirección General de Higiene y Seguridad Alimentaria. Laboratorio de Investigación y Monitoreo; Argentina | es_AR |
dc.description.fil | Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina | es_AR |
dc.subtype | cientifico |
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