Mostrar el registro sencillo del ítem
resumen
Resumen
Aims:
Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails.
Methods and Results:
We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of
[ver mas...]
dc.contributor.author | Piccinni, Florencia Elizabeth | |
dc.contributor.author | Ontañon, Ornella Mailén | |
dc.contributor.author | Ghio, Silvina | |
dc.contributor.author | Sauka, Diego Hernan | |
dc.contributor.author | Talia, Paola Mónica | |
dc.contributor.author | Rivarola, Maximo Lisandro | |
dc.contributor.author | Valacco, María Pía | |
dc.contributor.author | Campos, Eleonora | |
dc.date.accessioned | 2019-03-28T13:15:20Z | |
dc.date.available | 2019-03-28T13:15:20Z | |
dc.date.issued | 2018-12 | |
dc.identifier.issn | 1364-5072 | |
dc.identifier.other | https://doi.org/10.1111/jam.14176 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4769 | |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/full/10.1111/jam.14176 | |
dc.description.abstract | Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo‐ and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β‐xylosidase, a β‐glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose‐degrading enzymes applicable to bioprocessing and biotechnological industries. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.publisher | Wiley; Society for Applied Microbiology | |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Journal of applied microbiology 126 (3) : 811-825. (March 2019) | eng |
dc.subject | Cellulomonas | es_AR |
dc.subject | Glicosiltransferasas | es_AR |
dc.subject | Glycosyltransferases | eng |
dc.subject | Lignocellulose | eng |
dc.subject | Lignocelulosa | es_AR |
dc.subject.other | Secretome | eng |
dc.subject.other | Cazymes | eng |
dc.subject.other | GH10 | eng |
dc.subject.other | Glucanases | eng |
dc.subject.other | Glycosyl Hydrolases | eng |
dc.subject.other | Xylanases | eng |
dc.title | Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Piccinni, Florencia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina | es_AR |
dc.description.fil | Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Valacco, María Pía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; Argentina | es_AR |
dc.description.fil | Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
common
-
Artículos científicos [398]