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Pro-inflammatory cytokines (particularly IL-12) are important for initiating protective T helper 1 (Th1)-type immune responses and hence vital for combating intracellular infections and tumours. In situ hybridization (ISH) provides a powerful diagnostic tool allowing the identification and localization of cells producing these mediators in fixed tissues. The objective of this work was to produce a bovine IL-12p40 probe that allows detection of IL-12p40
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dc.contributor.author | Canton, German Jose | |
dc.contributor.author | Bartley, Paul M. | |
dc.contributor.author | Bartley, Kathryn | |
dc.contributor.author | Todd, Helen | |
dc.contributor.author | Chianini, Francesca | |
dc.contributor.author | Katzer, Frank | |
dc.date.accessioned | 2019-01-24T14:34:21Z | |
dc.date.available | 2019-01-24T14:34:21Z | |
dc.date.issued | 2013-02-15 | |
dc.identifier.issn | 0165-2427 | |
dc.identifier.issn | 1873-2534 | |
dc.identifier.other | https://doi.org/10.1016/j.vetimm.2012.11.019 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0165242712004242 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4327 | |
dc.description.abstract | Pro-inflammatory cytokines (particularly IL-12) are important for initiating protective T helper 1 (Th1)-type immune responses and hence vital for combating intracellular infections and tumours. In situ hybridization (ISH) provides a powerful diagnostic tool allowing the identification and localization of cells producing these mediators in fixed tissues. The objective of this work was to produce a bovine IL-12p40 probe that allows detection of IL-12p40 mRNA in fixed tissues from different ruminant species. The RNA probe sequence is 447 bp in length and from a region with high cross-species-sequence homology (>97.3% homology) to the ovine, cervine, caprine and bubaline IL-12p40 genes. ISH was carried out on paraformaldehyde fixed tissues collected from cattle, sheep and goats. The probe was efficient in identifying IL-12p40 expressing cells in fixed tissues from all these species. In conclusion, the IL-12p40 probe was efficient in identifying and localizing cells that express IL-12p40, and provides a good immuno-diagnostic technique to characterize immune responses in fixed tissues. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Elsevier | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Veterinary Immunology and Immunopathology 151 (3–4) : 342-347 (February 2013) | es_AR |
dc.subject | Rumiante | es_AR |
dc.subject | Ruminants | eng |
dc.subject | Citoquinas | es_AR |
dc.subject | Cytokines | eng |
dc.subject | Hibridación | es_AR |
dc.subject | Hybridization | eng |
dc.subject | Respuesta Inmunológica | es_AR |
dc.subject | Immune Response | eng |
dc.subject | Análisis de Tejidos | es_AR |
dc.subject | Tissue Analysis | eng |
dc.title | Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | EEA Balcarce | es_AR |
dc.description.fil | Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina | es_AR |
dc.description.fil | Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido | es_AR |
dc.description.fil | Fil: Bartley, Kathryn. Moredun Research Institute; Reino Unido | es_AR |
dc.description.fil | Fil: Todd, Helen. Moredun Research Institute; Reino Unido | es_AR |
dc.description.fil | Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido | es_AR |
dc.description.fil | Fil: Katzer, Frank. Moredun Research Institute; Reino Unido | es_AR |
dc.subtype | cientifico |
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