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Background: Several types and subtypes of bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, making type/subtype differentiation essential to understand the pathogenesis and epidemiology of BoHV infections. BoHV-5 subtyping is currently carried out by BstEII restriction enzyme analysis (REA) of the complete virus genome. This method allowed the description of three subtypes, one of which is
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dc.contributor.author | Maidana, Silvina Soledad | |
dc.contributor.author | Morano, Cintia Débora | |
dc.contributor.author | Cianfrini, Daniela | |
dc.contributor.author | Campos, Fabrício Souza | |
dc.contributor.author | Roehe, Paulo Michel | |
dc.contributor.author | Siedler, Bianca | |
dc.contributor.author | De Stefano, Gabriel Alejandro | |
dc.contributor.author | Mauroy, Axel | |
dc.contributor.author | Thiry, Etienne | |
dc.contributor.author | Romera, Sonia Alejandra | |
dc.date.accessioned | 2019-01-16T15:24:20Z | |
dc.date.available | 2019-01-16T15:24:20Z | |
dc.date.issued | 2013-06 | |
dc.identifier.issn | 1746-6148 | |
dc.identifier.other | https://doi.org/10.1186/1746-6148-9-111 | |
dc.identifier.uri | https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-9-111 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4276 | |
dc.description.abstract | Background: Several types and subtypes of bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, making type/subtype differentiation essential to understand the pathogenesis and epidemiology of BoHV infections. BoHV-5 subtyping is currently carried out by BstEII restriction enzyme analysis (REA) of the complete virus genome. This method allowed the description of three subtypes, one of which is the most widespread while the remaining two have so far only been found in South America. The present work describes a multiplex PCR followed by REA for BoHV-5 subtyping. Results: The method consists in the simultaneous amplification of glycoprotein B and UL54 gene fragments of 534 and 669 base pairs (bp), respectively, BstEII digestion of amplicons, separation of products in 1% agarose gels, and analysis of fragment length polymorphims. The multiplex PCR detected up to 227 BoHV-5 genome copies and 9.2 × 105 BoHV-5 genome copies when DNA was extracted from purified virus or infected tissue homogenates, respectively. The applicability of multiplex PCR-REA was demonstrated on 3 BoHV-5 reference strains. In addition, subtyping of two new isolates and seventeen previously reported ones (17 BHV-5a and 2 BHV-5b) by this method gave coincident results with those obtained with the classic BstEII REA assay. Conclusions: Multiplex PCR-REA provides a new tool for the fast and simple diagnosis and subtyping of BoHV-5. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | BMC | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | BMC Veterinary Research 39 : 111 (2013) | es_AR |
dc.subject | Enfermedades de los Animales | es_AR |
dc.subject | Animal Diseases | eng |
dc.subject | Ganado Bovino | es_AR |
dc.subject | Cattle | eng |
dc.subject | Virus de los Animales | es_AR |
dc.subject | Animal Viruses | eng |
dc.subject | Herpesviridae | es_AR |
dc.subject | PCR | es_AR |
dc.subject | Polimorfismo | es_AR |
dc.subject | Polymorphism | eng |
dc.subject | Herpes Virus Bovino | es_AR |
dc.subject | Bovine Herpesvirus | eng |
dc.subject.other | Herpesvirus | es_AR |
dc.subject.other | Reacción en Cadena de la Polimerasa | es_AR |
dc.title | Multiplex PCR followed by restriction length polymorphism analysis for the subtyping of bovine herpesvirus 5 isolates | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Virología | es_AR |
dc.description.fil | Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Morano, Cintia Débora Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. | es_AR |
dc.description.fil | Fil: Cianfrini, Daniela. Tecnovax SA; Argentina | es_AR |
dc.description.fil | Fil: Campos, Fabrício Souza. Universidade Federal do Rio Grande do Sul. Institute of Basic Health Sciences. Department of Microbiology, Immunology and Parasitology. Virology Laboratory; Brasil | es_AR |
dc.description.fil | Fil: Roehe, Paulo Michel. Universidade Federal do Rio Grande do Sul. Institute of Basic Health Sciences. Department of Microbiology, Immunology and Parasitology. Virology Laboratory; Brasil | es_AR |
dc.description.fil | Fil: Siedler, Bianca. Universidade Federal de Pelotas. Laboratório de Bioprocessos; Brasil | es_AR |
dc.description.fil | Fil: De Stefano, Gabriel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Mauroy, Axel. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica | es_AR |
dc.description.fil | Fil: Thiry, Etienne. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica | es_AR |
dc.description.fil | Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina | es_AR |
dc.subtype | cientifico |
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