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Resumen
The large-scale production of nematophagous fungi as agents of biological control is one of the main challenges to be commercially used. In order to improve growth of microorganism in a culture medium, the addition of growth inducer is common. At the moment, the action of their addition in the mycelia growth and sporulation rate of nematophagous fungi is not known. The purpose of this trial was to evaluate the sporulation rate of Duddingtonia flagrans by
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dc.contributor.author | Sagüés, María Federica | |
dc.contributor.author | Fusé, Luis Alberto | |
dc.contributor.author | Iglesias, Lucía Emilia | |
dc.contributor.author | Moreno, Fabiana Carina | |
dc.contributor.author | Saumell, Carlos Alfredo | |
dc.date.accessioned | 2018-12-04T12:04:42Z | |
dc.date.available | 2018-12-04T12:04:42Z | |
dc.date.issued | 2013-03 | |
dc.identifier.issn | 0932-0113 | |
dc.identifier.issn | 1432-1955 | |
dc.identifier.other | https://doi.org/10.1007/s00436-012-3231-0 | |
dc.identifier.uri | https://link.springer.com/article/10.1007/s00436-012-3231-0 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/4004 | |
dc.description.abstract | The large-scale production of nematophagous fungi as agents of biological control is one of the main challenges to be commercially used. In order to improve growth of microorganism in a culture medium, the addition of growth inducer is common. At the moment, the action of their addition in the mycelia growth and sporulation rate of nematophagous fungi is not known. The purpose of this trial was to evaluate the sporulation rate of Duddingtonia flagrans by adding two growth inducers, meso-inositol and Tween 80, both at 0.5 % in a traditional culture medium Sabouraud glucose agar (SGA) and also in a traditional culture medium enriched with wheat flour and milk powder. From a traditional sterile culture of D. flagrans, four groups were made: SGA; Sabouraud glucose agar–meso-inositol 0.5 %; Sabouraud glucose agar–Tween 80 0.5 %; and Sabouraud glucose agar-enriched (SGA-E). These media were placed at a constant temperature of 27 °C for 4 weeks. Following this, chlamydospores were gently rinsed off with sterile water and counted using a Neubauer haematocytometer to estimate the number of chlamydospores per millilitre of water. The addition of meso-inositol 0.5 % to SGA promoted a significant increase (p < 0.05) in chlamydospore production obtaining an average of 51,715,000 chlamydospores per Petri dish. The highest chlamydospore concentration was observed in the SGA-E in comparison with SGA (p < 0.01) obtaining an average of 208,760,000 chlamydospores. The aim of this study was to obtain basic knowledge regarding the effect of enriched culture medium and growth-inducing meso-inositol and Tween 80 on mycelial growth and production of chlamydospores. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Springer | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Parasitology Research 112 (3) : 1047–1051 (March 2013) | es_AR |
dc.subject | Clamidosporas | es_AR |
dc.subject | Chlamydospores | eng |
dc.subject | Hongos Nematófagos | es_AR |
dc.subject | Nematophagous Fungi | eng |
dc.subject | Control Biológico | es_AR |
dc.subject | Biological Control | eng |
dc.subject | Experimentación en Laboratorio | es_AR |
dc.subject | Laboratory Experimentation | eng |
dc.subject | Nematoda | es_AR |
dc.subject | Medio de Cultivo | es_AR |
dc.subject | Culture Media | eng |
dc.subject.other | Duddingtonia flagrans | es_AR |
dc.subject.other | Nematodes Gastrointestinales | es_AR |
dc.title | Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | EEA Balcarce | es_AR |
dc.description.fil | Fil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina | es_AR |
dc.description.fil | Fil: Fusé, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina | es_AR |
dc.description.fil | Fil: Iglesias, Lucía Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina | es_AR |
dc.description.fil | Fil: Moreno, Fabiana Carina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina | es_AR |
dc.description.fil | Fil: Saumell, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina | es_AR |
dc.subtype | cientifico |
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