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Non-cytopathic (ncp) type 2 bovine viral diarrhea virus (BVDV-2) is widely prevalent in Argentina causing high mortality rates in cattle herds. In this study, we characterized an Argentinean ncp BVDV-2 field isolate (98-124) compared to a high-virulence reference strain (NY-93), using in silico analysis, in vitro assays, and in vivo infections of colostrum-deprived calves (CDC) to compare pathogenic characters and virulence. In vitro infection of bovine
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dc.contributor.author | Malacari, Darío Amilcar | |
dc.contributor.author | Pecora, Andrea | |
dc.contributor.author | Perez Aguirreburialde, Maria Sol | |
dc.contributor.author | Cardoso, Nancy Patricia | |
dc.contributor.author | Odeon, Anselmo Carlos | |
dc.contributor.author | Capozzo, Alejandra Victoria | |
dc.date.accessioned | 2018-11-05T11:02:20Z | |
dc.date.available | 2018-11-05T11:02:20Z | |
dc.date.issued | 2018-04 | |
dc.identifier.issn | 2297-1769 | |
dc.identifier.other | https://doi.org/10.3389/fvets.2018.00075 | |
dc.identifier.uri | https://www.frontiersin.org/articles/10.3389/fvets.2018.00075/full | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/3775 | |
dc.description.abstract | Non-cytopathic (ncp) type 2 bovine viral diarrhea virus (BVDV-2) is widely prevalent in Argentina causing high mortality rates in cattle herds. In this study, we characterized an Argentinean ncp BVDV-2 field isolate (98-124) compared to a high-virulence reference strain (NY-93), using in silico analysis, in vitro assays, and in vivo infections of colostrum-deprived calves (CDC) to compare pathogenic characters and virulence. In vitro infection of bovine peripheral blood mononuclear cells (PBMC) with BVDV 98-124 induced necrosis shortly after infection while NY-93 strain increased the apoptotic rate in infected cells. Experimental infection of CDC (n = 4 each) with these strains caused an enteric syndrome. High pyrexia was detected in both groups. Viremia and shedding were more prolonged in the CDC infected with the NY-93 strain. In addition, NY-93 infection elicited a severe lymphopenia that lasted for 14 days, whereas 98-124 strain reduced the leukocyte counts for 5 days. All infected animals had a diminished lymphoproliferation activity in response to a mitogen. Neutralizing and anti-NS3 antibodies were detected 3 weeks after infection in all infected calves. Virulence was associated with a more severe clinical score, prolonged immune-suppression, and a greater window for transmission. Studies of apoptosis/necrosis performed after in vitro PBMC infection also revealed differences between both strains that might be correlated to the in vivo pathogenesis. Our results identified 98-124 as a low-virulence strain. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Frontiers Media | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/PNSA/1115053/AR./Biotecnologías reproductivas y desarrollo de metodologías de diagnóstico, control y prevención de las enfermedades infecciosas y parasitarias que afectan la concepción, gestación y período neonatal en especies de interés zootécnico. | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Frontiers in Veterinary Science 5 : 75 (Abril 2018) | es_AR |
dc.subject | Bovine Diarrhoea Pestivirus | eng |
dc.subject | Pestivirus de la Diarrea Bovina | es_AR |
dc.subject | Pathogenicity | eng |
dc.subject | Patogenicidad | es_AR |
dc.subject | Virulence | eng |
dc.subject | Virulencia | es_AR |
dc.subject | Experimental Infection | eng |
dc.subject | Infección Experimental | es_AR |
dc.subject | Calves | eng |
dc.subject | Ternero | es_AR |
dc.subject | Argentina | es_AR |
dc.title | In vitro and in vivo characterization of a typical and a high pathogenic bovine viral diarrhea virus type ii strains | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Virología | es_AR |
dc.description.fil | Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Cardoso, Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina | es_AR |
dc.description.fil | Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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