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Mostrando ítems 1-13 de 13
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A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos
Canizo, Jésica Romina; Ynsaurralde Rivolta, Amanda Eugenia; Vazquez Echegaray, Camila; Suvá, Mariana; Alberio, Virgilia; Aller Atucha, Juan Florencio; Guberman, Alejandra; Salamone, Daniel Felipe; Alberio, Ricardo; Alberio, Ramiro (BioMed Central, 2019-07-04)Background: The segregation of the hypoblast and the emergence of the pluripotent epiblast mark the final stages of blastocyst formation in mammalian embryos. In bovine embryos the formation of the hypoblast has been ... -
Calidad morfológica de ovocitos obtenidos in vivo en diferentes ondas foliculares de vacas tratadas con FSH
Aller Atucha, Juan Florencio; Callejas, Santiago; Alberio, Ricardo (Ediciones Taurus, 2020-08)Los objetivos del presente trabajo fueron determinar 1) el efecto de un tratamiento con FSH y 2) el período del ciclo estral (metaestro= primera onda folicular o diestro= segunda onda folicular) durante el crecimiento de ... -
Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
Manes, Jorgelina; Fiorentino, María Andrea; Kaiser, German Gustavo; Hozbor, Federico Andres; Alberio, Ricardo; Sánchez, Esteban O.; Paolicchi, Fernando (Elsevier, 2010-11)The objective of this study was to characterize the vaginal bacterial flora and subsequent pregnancy rates after AI in sheep subjected to 4 different estrous synchronization regimes – the use of two intravaginal devices ... -
Combined epidermal growth factor and hyaluronic acid supplementation of in vitro maturation medium and its impact on bovine oocyte proteome and competence
Rios, Glenda Laura; Buschiazzo, Jorgelina; Mucci, Nicolas Crescencio; Kaiser, German Gustavo; Cesari, Andreina; Alberio, Ricardo (Elsevier, 2015-03-15)The conditions for in vitro oocyte maturation impact on cytoplasmic and nuclear processes in the oocyte. These events are differentially influenced by the nature of the maturation inducer and the presence of intact cumulus ... -
Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
González, Noelia; Gil, María Lydia; Rios, Glenda Laura; Canizo, Jésica Romina; De Blas, José Ignacio; Alberio, Ricardo (Bentham Open, 2014-02)The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from ... -
Effect of Seminal Plasma on Post‐Thaw Quality and Functionality of Corriedale Ram Sperm Obtained by Electroejaculation and Artificial Vagina
Ledesma, Alba; Manes, Jorgelina; Rios, Glenda Laura; Aller Atucha, Juan Florencio; Cesari, Andreina; Alberio, Ricardo; Hozbor, Federico Andres (Wiley, 2015-06)We have already shown that seminal collection method affects seminal plasma composition and sperm quality in Corriedale rams. In this study, we evaluated the effect of seminal plasma collected by electroejaculation or ... -
Electroejaculation Increases Low Molecular Weight Proteins in Seminal Plasma Modifying Sperm Quality in Corriedale Rams
Ledesma, Alba; Manes, Jorgelina; Cesari, Andreina; Alberio, Ricardo; Hozbor, Federico Andres (Wiley, 2014-04)This study was conducted to evaluate the effect of seminal collection method (artificial vagina or electroejaculation) on the protein composition of seminal plasma and sperm quality parameters in Corriedale rams. To address ... -
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
Canizo, Jésica Romina; Vazquez Echegaray, Camila; Klisch, Doris; Aller Atucha, Juan Florencio; Paz, Dante; Alberio, Ricardo; Alberio, Ramiro; Guberman, Alejandra (BioMedCentral, 2018-07-27)OBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this ... -
Free cholesterol and cholesterol esters in bovine oocytes: Implications in survival and membrane raft organization after cryopreservation
Buschiazzo, Jorgelina; Rios, Glenda Laura; Canizo, Jésica Romina; Antollini, Silvia Susana; Alberio, Ricardo (2017)Part of the damage caused by cryopreservation of mammalian oocytes occurs at the plasma membrane. The addition of cholesterol to cell membranes as a strategy to make it more tolerant to cryopreservation has been little ... -
Improve intra‐uterine insemination in rabbits using ultra‐high temperature skim milk as extender to keep semen at room temperature
Hozbor, Federico Andres; Ledesma, Alba; Manes, Jorgelina; Rios, Glenda Laura; Kaiser, German Gustavo; Cano, Adriana Virginia; Luciano, Cecilia Ines; Alberio, Ricardo (Wiley, 2016-03)Two experiments were carried out to examine in vitro quality and in vivo fertility of rabbit semen diluted in ultra‐high temperature (UHT) skim milk. In the first experiment, pooled ejaculates of 10 adult rabbits were ... -
Linoleic acid stimulates neutral lipid accumulation in lipid droplets of maturing bovine oocytes
Carro, María De Las Mercedes; Buschiazzo, Jorgelina; Rios, Glenda Laura; Oresti, Gerardo Martin; Alberio, Ricardo (Elsevier, 2013-03-01)Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in bovine follicular fluid; when added to maturation culture media, it affects oocyte competence (depending on the type and concentration ... -
Llama (Lama glama) semen collection via thermo-electric artificial vagina: effect of seasonality and collection interval on ejaculate characteristics
Ferre, Luis Bernardo; Malik, Genoveva; Aller Atucha, Juan Florencio; Alberio, Ricardo; Fresno, Cristóbal; Kjelland, Michael (Elsevier, 2015-09-09)Adult male Llamas (Lama glama) (n = 6) were collected using a receptive female and a thermo-electric artificial vagina assembled with a polyethylene collection bag with the following objectives: (a) to develop a reliable ... -
Vaginal histological changes after using intravaginal sponges for oestrous synchronization in anoestrous ewes
Manes, Jorgelina; Campero, Carlos Manuel; Hozbor, Federico Andres; Alberio, Ricardo; Ungerfeld, Rodolfo (Wiley, 2015-04)To characterize the histological and cytological vaginal changes generated by the use of intravaginal sponge (IS) applied in oestrous synchronization treatments in ewes during mid‐non‐breeding season. Thirty‐five multiparous ...