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Mostrando ítems 1-20 de 21
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A thermostable GH8 endoglucanase of Enterobacter sp. R1 is suitable for β- glucan deconstruction
Ontañon, Ornella Mailen; Ghio, Silvina; Marrero Diaz De Villegas, Rubén; Garrido, Mercedes Maria; Talia, Paola Mónica; Fehér, Csaba; Campos, Eleonora (Elsevier, 2019-11)Glycoside hydrolase family 8 (GH8) includes endoglucanases, lichenases, chitosanases and xylanases, which are essential for polysaccharides breakdown. In this work, we studied a thermally stable GH8 from the cellulose ... -
Application of In-house xylanases as an addition to a commercial cellulase cocktail for the sustainable saccharification of pretreated blue agave bagasse used for bioethanol production
Montiel, Carmina; Hernández-Meléndez, Oscar; Marques, Susana; Gírio, Francisco; Tavares, João; Ontañon, Ornella Mailen; Campos, Eleonora; Bárzana, Eduardo (MDPI, 2024-08)The study involves the use of commercial cellulase Cellic CTec2 in combination with two in-house xylanases, PxXyn10A (XynA), a recombinant purified enzyme from Paenibacillus xylanivorans A59, and a xylanase enzymatic extract ... -
Characterization of a novel GH10 alkali‑thermostable xylanase from a termite microbiome
Mon, Maria Laura; Marrero Diaz De Vill, Rubén; Campos, Eleonora; Soria, Marcelo Abel; Talia, Paola Mónica (Springer Open, 2022-08)The aim of the present study was to assess the biochemical and molecular structural characteristics of a novel alkali-thermostable GH10 xylanase (Xyl10B) identified in a termite gut microbiome by a shotgun metagenomic ... -
Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
Romano, Nelson Gaston; Gioffre, Andrea; Sede, Silvana Mabel; Campos, Eleonora; Cataldi, Angel Adrian; Talia, Paola Mónica (Springer, 2013-08)Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups ... -
Characterization of two GH5 endoglucanases from termite microbiome using synthetic metagenomics
Ben Guerrero, Emiliano; Marrero Diaz De Villegas, Ruben; Soria, Marcelo Abel; Santangelo, María De La Paz; Campos, Eleonora; Talia, Paola Mónica (Springer, 2020-08)Here, we characterize two novel GH5 endoglucanases (GH5CelA and GH5CelB) from an uncultured bacterium identified in termite gut microbiomes. Both genes were codon-optimized, synthetized, cloned, and expressed as recombinant ... -
Draft Genome Sequence of Cellulolytic and Xylanolytic Cellulomonas sp. Strain B6 Isolated from Subtropical Forest Soil
Piccinni, Florencia Elizabeth; Murua, Yanina; Ghio, Silvina; Talia, Paola Mónica; Rivarola, Maximo Lisandro; Campos, Eleonora (2016-08)The genome information will be useful for studies of microbial enzymes for industrial application in lignocellulosic biomass utilization. -
EcXyl43 β-xylosidase: molecular modeling, activity on natural and artificial substrates, and synergism with endoxylanases for lignocellulose deconstruction
Ontañon, Ornella Mailén; Ghio, Silvina; Marrero Diaz De Vill, Rubén; Piccinni, Florencia Elizabeth; Talia, Paola Mónica; Cerutti, Maria Laura; Campos, Eleonora (Springer, 2018-08)Biomass hydrolysis constitutes a bottleneck for the biotransformation of lignocellulosic residues into bioethanol and high-value products. The efficient deconstruction of polysaccharides to fermentable sugars requires ... -
Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
Capetti, Caio; Arnoldi Pellegrini, Vanessa; Vacilotto, Milena Moreira; da Silva Curvelo, Antonio Aprigio; Falvo, Maurício; Guimaraes, Francisco E.G.; Ontañon, Ornella Mailen; Campos, Eleonora; Polikarpov, Igor (Springer, 2023-10)Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are ... -
Heterologous production and characterization of a thermostable GH10 family endo-xylanase from Pycnoporus sanguineus BAFC 2126
Niderhaus, Cecilia; Garrido, Mercedes Maria; Insani, Ester Marina; Campos, Eleonora; Wirth, Sonia Alejandra (Elsevier, 2018-04)Xylanases are key enzymes for agricultural biomass saccharification for the production of cellulosic ethanol. Success in enzymatic lignocellulose bioconversion is restricted by enzyme production costs, activity and stability ... -
Insights into the xylan degradation system of Cellulomonas sp. B6 : biochemical characterization of rCsXyn10A and rCsAbf62A
Garrido, Mercedes Maria; Piccinni, Florencia Elizabeth; Landoni, Malena; Peña, María Jesús; Topalian, Juliana; Couto, Alicia; Wirth, Sonia Alejandra; Urbanowicz, Breeanna Rae; Campos, Eleonora (Springer, 2022)Valorization of the hemicellulose fraction of plant biomass is crucial for the sustainability of lignocellulosic biorefineries. The Cellulomonas genus comprises Gram-positive Actinobacteria that degrade cellulose and other ... -
Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
Romero Victorica, Matias; Soria, Marcelo Abel; Batista-García, Ramón Alberto; Ceja-Navarro, Javier A.; Vikram, Surendra; Ortiz, Maximiliano; Ontañon, Ornella; Ghio, Silvina; Martínez-Ávila, Liliana; Quintero García, Omar Jasiel; Etcheverry, Clara; Campos, Eleonora; Cowan, Donald; Arneodo Larochette, Joel Demian; Talia, Paola Mónica (Springer Nature, 2020-03-02)In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different ... -
Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
Ontañon, Ornella Mailen; Bedő, Soma; Ghio, Silvina; Garrido, Mercedes Maria; Topalian, Juliana; Jahola, Dóra; Fehér, Anikó; Valacco, Maria Pia; Campos, Eleonora; Fehér, Csaba (Springer, 2021-05)One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still ... -
Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion
Coniglio, Romina O.; Fonseca, María I.; Diaz, Gabriela V.; Ontañon, Ornella Mailén; Ghio, Silvina; Campos, Eleonora; Zapata, Pedro Darío (Taylor & Francis, 2019-04)The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing ... -
Paenibacillus xylanivorans sp. nov., a xylan-degrading bacterium isolated from decaying forest soil
Ghio, Silvina; Sauka, Diego Hernan; Ferrari, Alejandro E.; Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Campos, Eleonora (Microbiology Society, 2019-12)A xylanolytic bacterial strain, named A59T, was isolated from a forest soil consortium in southern Argentina. Strain A59T is a Gram-stain-positive, facultative anaerobic, endospore-forming and rod-shaped bacterium. Its ... -
Production of a bacterial secretome highly efficient for the deconstruction of xylans
Topalian, Juliana; Navas, Laura Emilce; Ontañon, Ornella Mailen; Valacco, Maria Pia; Noseda, Diego Gabriel; Blasco, Martín; Peña, María Jesús; Urbanowicz, Breeanna Rae; Campos, Eleonora (Springer, 2024-09)Bacteria within the Paenibacillus genus are known to secrete a diverse array of enzymes capable of breaking down plant cell wall polysaccharides. We studied the extracellular xylanolytic activity of Paenibacillus xylanivorans ... -
PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus
Garrido, Mercedes Maria; Landoni, Malena; Sabbadin, Federico; Valacco, María Pía; Couto, Alicia; Bruce, Neil Charles; Wirth, Sonia Alejandra; Campos, Eleonora (Springer, 2020-09)Woody biomass represents an important source of carbon on earth, and its global recycling is highly dependent on Agaricomycetes fungi. White-rot Basidiomycetes are a very important group in this regard, as they possess a ... -
Purification and characterization of a GH43 β-xylosidase from Enterobacter sp. identified and cloned from forest soil bacteria
Campos, Eleonora; Negro Alvarez, María José; Sabaris Di Lorenzo, Gonzalo Julián; Gonzalez, Sergio Alberto; Rorig, Marcela Laura; Talia, Paola Mónica; Grasso, Daniel Horacio; Sáez, Felicia; Manzanares Secades, Paloma; Ballesteros Perdices, Mercedes; Cataldi, Angel Adrian (Elsevier, 2014-03)The use of lignocellulosic biomass for second generation biofuels requires optimization of enzymatic breakdown of plant cell walls. In this work, cellulolytic bacteria were isolated from a native and two cultivated forest ... -
Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Ghio, Silvina; Sauka, Diego Hernan; Talia, Paola Mónica; Rivarola, Maximo Lisandro; Valacco, María Pía; Campos, Eleonora (Wiley; Society for Applied Microbiology, 2018-12)Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual ... -
Structural and molecular dynamics investigations of ligand stabilization via secondary binding site interactions in Paenibacillus xylanivorans GH11 xylanase
Briganti, Lorenzo; Capetti, Caio; Pellegrini, Vanessa O. A.; Ghio, Silvina; Campos, Eleonora; Nascimento, Alessandro S.; Polikarpov, Igor (Elsevier, 2021-03)Glycoside hydrolases (GHs) are essential for plant biomass deconstruction. GH11 family consist of endo-β-1,4-xylanases which hydrolyze xylan, the second most abundant cell wall biopolymer after cellulose, into small ... -
Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversio
Ghio, Silvina; Bradanini, María B.; Garrido, Mercedes Maria; Ontañon, Ornella Mailen; Piccinni, Florencia Elizabeth; Marrero Diaz De Vill, Rubén; Talia, Paola Mónica; Campos, Eleonora (Elsevier, 2020-12)In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase ...