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Mostrando ítems 1-16 de 16
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A prime-boost combination of a three-protein cocktail and multiepitopic MVA as a vaccine against Babesia bigemina elicits neutralizing antibodies and a Th1 cellular immune response in mice
Montenegro, Valeria Noely; Jaramillo Ortiz, Jose; Paoletta, Martina; Gravisaco, María José; Del Medico Zajac, Maria Paula; Garanzini, Debora Patricia; Valenzano, Magali Nicole; Calamante, Gabriela; Wilkowsky, Silvina Elizabeth (Elsevier, 2022-09)In the intraerythrocytic protozoan parasites of the genus Babesia both innate and adaptive immune responses are necessary to confer protection against clinical disease. In particular, the adaptive immune response involves ... -
An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
Wilkowsky, Silvina Elizabeth; Gimenez Bareiro, Guillermo; Mon, Maria Laura; Moore, Prando Dadin; Caspe, Sergio Gaston; Campero, Carlos Manuel; Fort, Marcelo Cristian; Romano, Maria Isabel (Sage Publishing, 2011-08)Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for ... -
Desarrollo de inmunocromatografías para el diagnóstico de leptospirosis y neosporosis bovina
Montenegro, Valeria Noely; Varni, Vanina Delia; Nagel, Ariel Gaston; Jaramillo Ortiz, Jose Manuel; Paoletta, Martina; De La Fourniere, Sofia Ana María; Macoretta, Christian Leandro; Moore, Prando Dadin; Brihuega, Bibiana Felicitas; Caimi, Karina Cynthia; Wilkowsky, Silvina Elizabeth (2016)Los abortos en el ganado bovino implican pérdidas económicas en la industria pecuaria; los agentes infecciosos son una de las principales causas asociadas. Entre las diversas enfermedades identificadas en nuestro país como ... -
Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
Guillemi, Eliana Carolina; Ruybal, Paula; Lia, Veronica Viviana; Gonzalez, Sergio Alberto; Lew, Sergio Eduardo; Zimmer, Patricia Andrea; Lopez Arias, Ludmila Sol; Rodríguez, José Luis; Rodriguez, Sonia Y.; Frutos, Roger; Wilkowsky, Silvina Elizabeth; Farber, Marisa Diana (Elsevier, 2015-03)Bovine Anaplasmosis caused by Anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where Rhipicephalus microplus is considered the most significant biological vector. Molecular markers ... -
Development of an Indirect ELISA Based on a Recombinant Chimeric Protein for the Detection of Antibodies against Bovine Babesiosis
Jaramillo Ortiz, Jose Manuel; Montenegro, Valeria Noely; De La Fourniere, Sofia Ana María; Sarmiento, Nestor Fabian; Farber, Marisa Diana; Wilkowsky, Silvina Elizabeth (2018)The current method for Babesia spp. serodiagnosis based on a crude merozoite antigen is a complex and time-consuming procedure. An indirect enzyme-linked immunosorbent assay (iELISA) based on a recombinant multi-antigen ... -
Development of highly sensitive one step-PCR tests for improved detection of B. bigemina and B. bovis
De La Fourniere, Sofía Ana María; Paoletta, Martina; Guillemi, Eliana Carolina; Sarmiento, Nestor Fabian; Donati, Pablo Alejandro; Wilkowsky, Silvina Elizabeth; Farber, Marisa Diana (Elsevier, 2021-08)Bovine babesiosis caused by Babesia bigemina and B. bovis is an economically relevant tick-borne disease distributed over tropical and subtropical world regions. Animals that recover from the clinical disease can remain ... -
Diversity of the BoLA-DRB3 gene in cattle breeds from tropical and subtropical regions of Argentina
Valenzano, Magali Nicole; Caffaro, María Eugenia; Lia, Veronica Viviana; Poli, Mario Andres; Wilkowsky, Silvina Elizabeth (Springer, 2021-12)Bovine leukocyte antigens (BoLA) have been widely studied because of their primary function in the recognition of pathogens by the immune system. To date, however, the characterization of the BoLA-DRB3 gene in Latin American ... -
Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay
Paoletta, Martina; Lopez Arias, Ludmila Sol; De La Fourniere, Sofia; Guillemi, Eliana Carolina; Luciani, Carlos; Sarmiento, Nestor Fabian; Mosqueda Gualito, Juan Joel; Farber, Marisa Diana; Wilkowsky, Silvina Elizabeth (2018-02)Vector-borne hemoparasitic infections are a major problem that affects livestock industries worldwide, particularly in tropical and subtropical regions. In this work, a reverse line blot (RLB) hybridization assay was ... -
Evaluation of different heterologous prime-boost immunization strategies against Babesia bovis using viral vectored and protein-adjuvant vaccines based on a chimeric multi-antigen
Jaramillo Ortiz, Jose Manuel; Molinari, Maria Paula; Gravisaco, María José; Paoletta, Martina; Montenegro, Valeria Noely; Wilkowsky, Silvina Elizabeth (2016)Protection against the intraerythrocytic bovine parasite Babesia bovis requires both humoral and cellular immune responses. Therefore, tailored combinations of immunogens targeted at both arms of the immune system are ... -
Identification and characterization of a Babesia bigemina thrombospondin-related superfamily member, TRAP-1 : a novel antigen containing neutralizing epitopes involved in merozoite invasion
Montenegro, Valeria Noely; Paoletta, Martina; Jaramillo Ortiz, Jose; Suarez, Carlos Esteban; Wilkowsky, Silvina Elizabeth (BioMed Central, 2020-12)Background: Thrombospondin-related anonymous protein (TRAP) has been described as a potential vaccine candidate for several diseases caused by apicomplexan parasites. However, this protein and members of this family have ... -
Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
Jaramillo Ortiz, Jose Manuel; Paoletta, Martina; Gravisaco, María José; Lopez Arias, Ludmila Sol; Montenegro, Valeria Noely; De La Fourniere, Sofia; Valenzano, Magali Nicole; Guillemi, Eliana Carolina; Valentini, Beatriz Susana; Echaide, Ignacio Eduardo; Farber, Marisa Diana; Wilkowsky, Silvina Elizabeth (Elsevier, 2019-10)Protection against the intraerythrocytic protozoan parasite Babesia bovis depends on both strong innate and adaptive immune response, this latter involving the presentation of parasite antigens to CD4+ T-lymphocytes by ... -
Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
Nuñez, Pablo Alfredo; Moretta, Rosalia Ester; Ruybal, Paula; Wilkowsky, Silvina Elizabeth; Farber, Marisa Diana (Springer, 2014-03)Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine ... -
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines
Guillemi, Eliana Carolina; De La Fourniere, Sofia Ana María; Orozco, Marcela; Peña Martínez, Jorge; Correa, Elena; Fernández, Javier; Lopez Arias, Ludmila Sol; Paoletta, Martina; Corona, Belkis; Pinarello, Valérie; Wilkowsky, Silvina Elizabeth; Farber, Marisa Diana (2016)Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, ... -
Novel biotechnological platform based on baculovirus occlusion bodies carrying Babesia bovis small antigenic peptides for the design of a diagnostic enzyme-linked immunosorbent assay (ELISA)
Lopez, Maria Gabriela; Pallares, H.M.; Alfonso, Victoria; Carmona, S. J.; Farber, Marisa Diana; Taboga, Oscar Alberto; Wilkowsky, Silvina Elizabeth (Springer, 2018-01)Baculoviruses are large DNA virus of insects principally employed in recombinant protein expression. Its ability to form occlusion bodies (OBs), which are composed mainly of polyhedrin protein (POLH), makes them ... -
The key to egress? Babesia bovis perforin-like protein 1 (PLP1) with hemolytic capacity is required for blood stage replication and is involved in the exit of the parasite from the host cell
Paoletta, Martina; Laughery, Jacob Michael; Lopez Arias, Ludmila Sol; Jaramillo Ortiz, Jose; Montenegro, Valeria Noely; Petrigh, Romina; Ueti, Massaro W.; Suarez, Carlos Esteban; Farber, Marisa Diana; Wilkowsky, Silvina Elizabeth (Elsevier, 2021-07)Bovine babesiosis is a tick-borne disease caused by apicomplexan parasites of the Babesia genus that represents a major constraint to livestock production worldwide. Currently available vaccines are based on live parasites ... -
Thrombospondin related anonymous protein superfamily in vector-borne apicomplexans: the parasite’s toolkit for cell invasion
Paoletta, Martina; Wilkowsky, Silvina Elizabeth (Frontiers Media, 2022-04)Apicomplexan parasites transmitted by vectors, including Babesia spp. and Plasmodium spp., cause severe disease in both humans and animals. These parasites have a complex life cycle during which they migrate, invade, and ...