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Mostrando ítems 1-20 de 30
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A fungal protease named AsES triggers antiviral immune responses and effectively restricts virus infection in arabidopsis and Nicotiana benthamiana plants
Caro, Maria Del Pilar; Venturuzzi, Andrea Laura; Moschen, Sebastian Nicolas; Salazar, Sergio Miguel; Díaz Ricci, Juan Carlos; Asurmendi, Sebastian (Oxford University Press, 2022-04)Background and Aims: Plants have evolved complex mechanisms to fight against pathogens. Among these mechanisms, pattern-triggered immunity (PTI) relies on the recognition of conserved microbe- or pathogen-associated molecular ... -
Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot and-mouth disease virus
Curra, Anabella Paola; Cacciabue, Marco Polo Domingo; Gravisaco, María José; Asurmendi, Sebastian; Taboga, Oscar Alberto; Gismondi, Maria Ines (Peer J Inc., 2021-06)RNA interference (RNAi) is a well-conserved mechanism in eukaryotic cells that directs post-transcriptional gene silencing through small RNA molecules. RNAi has been proposed as an alternative approach for rapid and specific ... -
Arabidopsis phenotyping through geometric morphometrics
Manacorda, Carlos Augusto; Asurmendi, Sebastian (Oxford University Press, 2018-07)Background: Recently, great technical progress has been achieved in the field of plant phenotyping. High-throughput platforms and the development of improved algorithms for rosette image segmentation make it possible to ... -
Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
Gismondi, Maria Ines; Ortiz, Xoana P.; Curra, Anabella Paola; Asurmendi, Sebastian; Taboga, Oscar Alberto (2014-04)RNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors ... -
Assessing small RNA profles in potato diploid hybrid and its resynthesized allopolyploid reveals conserved abundance with distinct genomic distribution
Zavallo, Diego; Cara, Nicolás; Leone, Melisa; Crescente, Juan Manuel; Marfil, Carlos Federico; Masuelli, Ricardo Williams; Asurmendi, Sebastian (Springer, 2024-04)Allopolyploidy, a complex process involving interspecific hybridization and whole genome duplication, significantly impacts plant evolution, leading to the emergence of novel phenotypes. Polyploids often present phenotypic ... -
Cestode parasites release extracellular vesicles with microRNAs and immunodiagnostic protein cargo
Ancarola, María Eugenia; Marcilla, Antonio; Herz, Michaela; Macchiaroli, Natalia; Pérez, Matías; Asurmendi, Sebastian; Brehm, Klaus; Poncini, Carolina; Rosenzvit, Mara Cecilia; Cucher, Marcela (2017-09)Intercellular communication is crucial in multiple aspects of cell biology. This interaction can be mediated by several mechanisms including extracellular vesicle (EV) transfer. EV secretion by parasites has been reported ... -
Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment
Marfil, Carlos Federico; Asurmendi, Sebastian; Masuelli, Ricardo Williams (2012-08)Phenotypic plasticity is often postulated as a principal characteristic of tuber-bearing wild Solanum species. The hypotheses to explore this observation have been developed based on the presence of genetic variation. In ... -
Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression
Reyes, Carina Andrea; Ocolotobiche, Eliana Evelina; Marmisollé, Facundo E.; Robles Luna, Gabriel; Bazzini, Ariel Alejandro; Asurmendi, Sebastian; García, María Laura (2016-04)Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. Here, we show that the infection ... -
Coinfection of cotton plants with cotton leafroll dwarf virus atypical and a novel cotton begomovirus
Delfosse, Veronica Cecilia; Debat, Humberto Julio; Zavallo, Diego; Alvarez, Liliana; Gomez Talquenca, Gonzalo; Bonacic Kresic, Iván; Asurmendi, Sebastian; Distefano, Ana Julia (Wiley, 2024-05)Cotton is a commercial crop of global importance whose versatile fibre is widely used in the textile industry. Cotton is vulnerable to infectious pathogens, including viruses, that are a major threat to cotton production. ... -
Coupling virus-induced gene silencing to exogenous green fluorescence protein expression provides a highly efficient system for functional genomics in arabidopsis and across all stages of tomato fruit development
Quadrana, Leandro Daniel; Rodriguez, Maria Cecilia; Bermudez Salazar, Luisa; Nunes Nesi, Adriano; Fernie, Alisdair R.; Descalzo, Adriana Maria; Asis, Ramón; Rossi, Magdalena; Asurmendi, Sebastian; Carrari, Fernando (2011-07)Since the advent of the postgenomic era, efforts have focused on the development of rapid strategies for annotating plant genes of unknown function. Given its simplicity and rapidity, virus-induced gene silencing (VIGS) ... -
Current recommendations and novel strategies for sustainable management of soybean sudden death syndrome
Rodriguez, Maria Cecilia; Sautua, Francisco José; Scandiani, María Mercedes; Carmona, Marcelo Anibal; Asurmendi, Sebastian (Wiley, 2021-10)The increase in food production requires reduction of the damage caused by plant pathogens, minimizing the environmental impact of management practices. Soil-borne pathogens are among the most relevant pathogens that affect ... -
Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level
Zavallo, Diego; Debat, Humberto Julio; Conti, Gabriela; Manacorda, Carlos Augusto; Rodriguez, Maria Cecilia; Asurmendi, Sebastian (2015-08-03)Small RNAs (sRNAs) play important roles in plant development and host-pathogen interactions. Several studies have highlighted the relationship between viral infections, endogenous sRNA accumulation and transcriptional ... -
Genome-wide identification of MITE-derived microRNAs and their targets in bread wheat
Crescente, Juan Manuel; Zavallo, Diego; Del Vas, Mariana; Asurmendi, Sebastian; Helguera, Marcelo; Fernandez, Elmer; Vanzetti, Leonardo Sebastian (BioMed Central, 2022-02)Background: Plant miRNAs are a class of small non-coding RNAs that can repress gene expression at the post-transcriptional level by targeting RNA degradation or promoting translational repression. There is increasing ... -
Genomic re-assessment of the transposable element landscape of the potato genome
Zavallo, Diego; Crescente, Juan Manuel; Gantuz, Magdalena; Leone, Melisa; Vanzetti, Leonardo Sebastian; Masuelli, Ricardo Williams; Asurmendi, Sebastian (Springer, 2020-05)Transposable elements (TEs) are DNA sequences with the ability to autoreplicate and move throughout the host genome. TEs are major drivers in stress response and genome evolution. Given their significance, the development ... -
Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements
Cambiagno, Damián Alejandro; Nota, María Florencia; Zavallo, Diego; Rius, Sebastián Pablo; Casati, Paula; Alvarez, Maria Elena; Asurmendi, Sebastian (Wiley, 2018-12)Pattern recognition receptors (PRR) and nucleotide‐binding leucine‐rich repeat proteins (NLR) are major components of the plant immune system responsible for pathogen detection. To date, the transcriptional regulation of ... -
Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector
De Haro, Luis Alejandro; Dumon, Analia Delina; Mattio, Maria Fernanda; Arguello Caro, Evangelina Beatriz; Llauger, Gabriela; Zavallo, Diego; Blanc, Hervé; Mongelli, Vanesa Claudia; Truol, Graciela Ana Maria; Saleh, María-Carla; Asurmendi, Sebastian; Del Vas, Mariana (2017)Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) ... -
Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
Bazzini, Ariel Alejandro; Manacorda, Carlos Augusto; Tohge, Takayuki; Conti, Gabriela; Rodriguez, Maria Cecilia; Nunes Nesi, Adriano; Villanueva, Sofía; Fernie, Alisdair R.; Carrari, Fernando; Asurmendi, Sebastian (2011-12-12)Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways ... -
MicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs
Pérez, Matías; Macchiaroli, Natalia; Lichtenstein, Gabriel; Conti, Gabriela; Asurmendi, Sebastian; Milone, Diego Humberto; Stegmayer, Georgina; Kamenetzky, Laura; Cucher, Marcela; Rosenzvit, Mara Cecilia (2017-09)MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth ... -
Modeling tobacco mosaic virus proliferation in protoplasts
Merchuk, Jose C.; Sánchez Mirón, Asterio; Asurmendi, Sebastian; Shacham, Mordechai (2016)The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically ... -
Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits
Dominguez, Pia Guadalupe; Conti, Gabriela; Duffy, Tomas; Insani, Ester Marina; Alseekh, Saleh; Asurmendi, Sebastian; Fernie, Alisdair R.; Carrari, Fernando (Oxford University Press, 2021-09)The transcription factor ASR1 (ABA, STRESS, RIPENING 1) plays multiple roles in plant responses to abiotic stresses as well as being involved in the regulation of central metabolism in several plant species. However, despite ...