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Resumen
Staphylococcus aureus is a worldwide pathogen that causes mastitis in dairy herds. Shortcomings in control programs have encouraged the development of vaccines against this pathogen. This study evaluated the vaccine candidate VacR, which included recombinant S. aureus protein clumping factor A (rClf), fibronectin binding protein A (rFnBP) and hemolysin beta (rBt), formulated with a novel immune-stimulating complex. Comparisons were made between healthy [ver mas...]
dc.contributor.authorPujato, Nazarena
dc.contributor.authorCamussone, Cecilia
dc.contributor.authorRenna, María Sol
dc.contributor.authorPerrig, Melina Soledad
dc.contributor.authorMorein, Bror
dc.contributor.authorCalvinho, Luis Fernando
dc.contributor.authorMarcipar, Iván Sergio
dc.date.accessioned2018-06-29T11:49:39Z
dc.date.available2018-06-29T11:49:39Z
dc.date.issued2018-05
dc.identifier.issn1090-0233
dc.identifier.otherhttps://doi.org/10.1016/j.tvjl.2018.03.005
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1090023318300686
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2694
dc.description.abstractStaphylococcus aureus is a worldwide pathogen that causes mastitis in dairy herds. Shortcomings in control programs have encouraged the development of vaccines against this pathogen. This study evaluated the vaccine candidate VacR, which included recombinant S. aureus protein clumping factor A (rClf), fibronectin binding protein A (rFnBP) and hemolysin beta (rBt), formulated with a novel immune-stimulating complex. Comparisons were made between healthy pregnant heifers that received either VacR (n = 8; VacR group) or phosphate buffered saline (PBS) plus adjuvant (control group) SC in the supramammary lymph node area on days 45 and 15 before the expected calving date. Blood and foremilk samples were collected from 7 to 60 days post-calving. After calving, heifers in the VacR group produced higher total IgG (IgGtotal) titers against each component, in both serum (rBt, 3.4 × 105; rClf, 3.1 × 105; rFnBP, 2.3 × 105) and milk (rBt, 2.6 × 104; rClf, 1.3 × 104; rFnBP, 1.1 × 104), than control heifers (P < 0.0001). There were increased concentrations of IgG1 and IgG2 in VacR group (P < 0.05), in both serum and milk. Humoral responses remained high throughout the period most susceptible to intramammary infections (P < 0.01). Antibodies produced against S. aureus rClf and rFnBP reduced bacterial adherence to fibronectin and fibrinogen by 73% and 67%, respectively (P < 0.001). Milk antibodies against these adhesins inhibited S. aureus invasion of a mammary epithelial cell line (MAC-T), resulting in 15.7% of bacteria internalized (P < 0.0001). There was an approximately 6-fold reduction in the hemolysis titer for the native hemolysin in the VacR group compared to the control group (P < 0.0001) and a significantly increase in the proportion of positive neutrophils (VacR, 29.7%; PBS, 13.1%) and the mean fluorescent index (VacR, 217.4; PBS, 152.6; P < 0.01) in the VacR group. The results suggest that VacR is a valuable vaccine candidate against S. aureus infections, and merits further field trials and experimental challenges.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceThe Veterinary Journal 235 : 47-53 (May 2018)es_AR
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectNovillaes_AR
dc.subjectHeiferseng
dc.subjectRespuesta Inmunológicaes_AR
dc.subjectImmune Responseeng
dc.subjectVacunaes_AR
dc.subjectVaccineseng
dc.subjectStaphylococcus aureuses_AR
dc.subjectMastítis Bovinaes_AR
dc.subjectBovine Mastitiseng
dc.subjectGestaciónes_AR
dc.subjectPregnancyeng
dc.titleEvaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heiferses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Rafaelaes_AR
dc.description.filFil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentinaes_AR
dc.description.filFil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Perrig, Melina Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina.es_AR
dc.description.filFil: Morein, Bror. Uppsala University. Department of Clinical Virology; Sueciaes_AR
dc.description.filFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Marcipar, Iván Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentinaes_AR
dc.subtypecientifico


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