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resumen

Resumen
Staphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from [ver mas...]
dc.contributor.authorÁgredo-Campos, Ángela Sofía
dc.contributor.authorCamussone, Cecilia
dc.contributor.authorAkineden, Ömer
dc.contributor.authorFernández-Silva, Jorge A.
dc.contributor.authorRamírez-Vásquez, Nicolás F.
dc.date.accessioned2025-03-05T13:55:50Z
dc.date.available2025-03-05T13:55:50Z
dc.date.issued2025-01
dc.identifier.issn0958-6946
dc.identifier.issn1879-0143
dc.identifier.otherhttps://doi.org/10.1016/j.idairyj.2024.106088
dc.identifier.urihttp://hdl.handle.net/20.500.12123/21541
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0958694624002085
dc.description.abstractStaphylococcus aureus is recognized for causing contagious mastitis in cattle. Elimination of the organism in affected animals is challenging due to its potential antibiotic resistance mechanisms and virulence factors. The aim of the study was the pheno- and genotypic epidemiological characterization of S. aureus isolated from bulk tank milk samples obtained from three municipalities in the northern region of Antioquia. Twenty-one S. aureus isolates from 150 bulk tank milk samples were characterized by evaluating antimicrobial susceptibility to 17 antibiotics using the VITEK 2, and identifying several genes encoding for virulence factors, enterotoxins, and the agr groups by conventional PCR. The clonal relationship between the isolates was assessed using macrorestriction fragment analysis (MRFA) of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). Most of the isolates showed susceptibility to the tested antibiotics; 19% exhibited resistance to tetracycline and all isolates showed beta-lactamases. Molecular typing revealed that 76% carried the agrI and cap5 genes. Seven isolates harbored genes coding for staphylococcal enterotoxins (SE), with seh being the most identified gene. Furthermore, MRFA demonstrated high heterogeneity among the isolates, resulting in the assignment of 18 different MRFA patterns. These results indicate phenotypic resistance to tetracycline and beta-lactams, the presence of genes encoding for staphylococcal enterotoxins, and high heterogeneity among S. aureus isolates from bulk tank milk.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/es_AR
dc.sourceInternational Dairy Journal 160 : 106088. (January 2025)es_AR
dc.subjectStaphylococcus aureuseng
dc.subjectEpidemiologíaes_AR
dc.subjectEpidemiologyeng
dc.subjectLechees_AR
dc.subjectMilkeng
dc.subjectFenologíaes_AR
dc.subjectPhenologyeng
dc.subjectMastítis Bovinaes_AR
dc.subjectBovine Mastitiseng
dc.subjectColombia
dc.titlePheno- and genotypic epidemiological characterization of Staphylococcus aureus isolated from bulk tank milk in Colombiaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)es_AR
dc.description.origenEEA Rafaelaes_AR
dc.description.filFil: Ágredo-Campos, Ángela Sofía. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombiaes_AR
dc.description.filFil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentinaes_AR
dc.description.filFil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentinaes_AR
dc.description.filFil: Akineden, Ömer. Justus-Liebig-University Giessen. Institute of Veterinary Food Science. Dairy Sciences; Alemaniaes_AR
dc.description.filFil: Fernández-Silva, Jorge A. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombiaes_AR
dc.description.filFil: Ramírez-Vásquez, Nicolás F. Universidad de Antioquia. Facultad de Ciencias Agrarias. Escuela de Medicina Veterinaria. Grupo Centauro; Colombiaes_AR
dc.subtypecientifico


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