Isolation and characterization of a Bacillus thurigiensis strain with potential for epizootics in Plodia interpunctella

Abstract

Bacillus thuringiensis is a gram-positive, spore-forming bacterium renowned for its production of pesticidal proteins, which make it highly effective against a wide range of insect pests. These proteins, primarily Cry and Vip, specifically target the gut epithelium of susceptible insects, causing feeding cessation and eventual death. As a result, B. thuringiensis has become a cornerstone of biological pest control, particularly in integrated pest management programs. Although widely used, B. thuringiensis is generally considered an opportunistic entomopathogen due to the rarity of natural epizootics. However, certain strains have been linked to significant pest mortality in specific environments. This study focused on isolating and characterizing B. thuringiensis strain INTA L404-1, which was associated with high mortality in an artificially reared population of Plodia interpunctella (Lepidoptera: Pyralidae), a significant pest of stored grains. By investigating its genetic profile, the phenotypic characteristics of its crystals, and its insecticidal properties, we aim to evaluate its potential as an effective biocontrol agent.