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A new method for simultaneous detection and discrimination of Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) using real time PCR with high resolution melting (HRM) analysis
Marin, Maia Solange; Quintana, Silvina; Leunda, Maria Rosa; Recavarren, Mariana Ines; Pagnuco, Inti Anabela; Spath, Ernesto Juan; Perez, Sandra; Odeon, Anselmo Carlos (Elsevier, 2016-01)Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are antigenically and genetically similar. The aim of this study was to develop a simple and reliable one-step real time PCR assay with high resolution melting (HRM) ...