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Resumen
Although studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a characterization of the cellulolytic activities of environmental mycobacterial isolates derived from soil and water samples from the central region of Argentina, aimed to evaluate the conservation of the mechanism for the degradation of cellulose
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dc.contributor.author | Mon, Maria Laura | |
dc.contributor.author | Romano, Nelson Gaston | |
dc.contributor.author | Farace, Pablo Daniel | |
dc.contributor.author | Tortone, Claudia Andrea | |
dc.contributor.author | Oriani, Delia Susana | |
dc.contributor.author | Picariello, Gianluca | |
dc.contributor.author | Zumarraga, Martin Jose | |
dc.contributor.author | Gioffre, Andrea | |
dc.contributor.author | Talia, Paola Mónica | |
dc.date.accessioned | 2024-05-14T17:55:15Z | |
dc.date.available | 2024-05-14T17:55:15Z | |
dc.date.issued | 2024-07 | |
dc.identifier.issn | 1472-9792 | |
dc.identifier.other | https://doi.org/10.1016/j.tube.2024.102516 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/17730 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/abs/pii/S1472979224000428 | |
dc.description.abstract | Although studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a characterization of the cellulolytic activities of environmental mycobacterial isolates derived from soil and water samples from the central region of Argentina, aimed to evaluate the conservation of the mechanism for the degradation of cellulose in this group of bacteria. The molecular and genomic identification revealed identity with Mycolicibacterium septicum. The endoglucanase and total cellulase activities were assessed both qualitatively and quantitatively and the optimal enzymatic conditions were characterized. A specific protein of around 56 kDa with cellulolytic activity was detected in a zymogram. Protein sequences possibly arising from a cellulase were identified by mass spectrometry-based shotgun proteomics. Results showed that M. septicum encodes for cellulose- and hemicellulose-related degrading enzymes, including at least an active β-1,4 endoglucanase enzyme that could be useful to improve its survival in the environment. Given the important health issues related to mycobacteria, the results of the present study may contribute to the knowledge of their cellulolytic system, which could be important for their ability to survive in many different types of environments. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Elsevier | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2023-PD-L01-I085, Identificación y caracterización funcional de genes interés biotecnológico para la sostenibilidad productiva y ambiental | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2023-PD-L01-I089, Microbiomas en ecosistemas agropecuarios: la conexión integradora del enfoque Una Salud | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2023-PD-L01-I102, Bases ecofisiológicas para mejorar la adaptación de los cultivos a la variabilidad ambiental mediante el manejo agronómico y el mejoramiento genético | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2023-PD-L04-I122, Gestión de las biomasas del SAB y estrategias tecnológicas para su transformación en bioproductos de valor agregado | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2019-PT-E7-I159-001, Info e innovación p/ VA, agroind. y bioenergía | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | es_AR |
dc.source | Tuberculosis 147 : 102516 (July 2024) | es_AR |
dc.subject | Cellulase | eng |
dc.subject | Celulasa | es_AR |
dc.subject | Cellulolytic Microorganisms | eng |
dc.subject | Microorganismos Celulolíticos | es_AR |
dc.subject.other | Mycolicibacterium septicum | es_AR |
dc.subject.other | Environmental Mycobacteria | eng |
dc.subject.other | Micobacterias Ambientales | es_AR |
dc.subject.other | Active Endoglucanase | eng |
dc.subject.other | Endoglucanasa Activa | es_AR |
dc.title | Exploring the cellulolytic activity of environmental mycobacteria | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | es_AR |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Romano, Nelson Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Romano, Nelson Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Farace, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Farace, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Cátedra de Bacteriología y Micología; Argentina | es_AR |
dc.description.fil | Fil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Cátedra de Bacteriología y Micología; Argentina | es_AR |
dc.description.fil | Fil: Picariello, Gianluca. Consiglio Nazionale Delle Ricerche. Istituto di Scienze Dell’Alimentazione; Italia | es_AR |
dc.description.fil | Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Gioffre, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Gioffre, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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