Instituto de Biotecnología: Envíos recientes
Mostrando ítems 281-300 de 437
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Trans-complementation of polyhedrin by a stably transformed Sf9 insect cell line allows occ− baculovirus occlusion and larval per os infectivity
(Elsevier, 2010-01-15)Oral infection of insect larvae with baculovirus is an advantageous methodology for producing high levels of recombinant proteins and for achieving plague control. However, many recombinant baculoviruses express a foreign ... -
Experimental selection of long‐term intracellular mycobacteria
(Wiley, 2014-09)Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo ... -
Detection of Mycobacterium bovis in Bovine and Bubaline tissues using Nested-PCR for TbD1
(PLOS, 2014-03)In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. ... -
Developments in plant-based vaccines against diseases of concern in developing countries
(BENTHAM Open, 2010)Vaccination has been extensively used to prevent, eradicate and control infectious diseases. However, the development and implementation of new vaccines remains prohibitively expensive for middle and low income countries where ... -
Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
(Oxford University Press, 2018-08)Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and ... -
A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants
(Oxford University Press, 2018-11)Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol ... -
An inhibitory mechanism of action of coiled‐coil peptides against type three secretion system from enteropathogenic Escherichia coli
(Wiley, 2019-03)Human pathogenic gram‐negative bacteria, such as enteropathogenic Escherichia coli (EPEC), rely on type III secretion systems (T3SS) to translocate virulence factors directly into host cells. The coiled‐coil domains present ... -
Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
(BMC, 2019-03)Background; Mal de Río Cuarto virus (MRCV) infects several monocotyledonous species including maize and wheat. Infected plants show shortened internodes, partial sterility, increased tillering and reduced root length. To ... -
Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
(Wiley; Society for Applied Microbiology, 2018-12)Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual ... -
Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
(PLOS, 2015-06-01)The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest ... -
Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato
(PLOS, 2019-03-25)Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action ... -
Tomato Abscisic Acid Stress ripening (ASR) gene family revisited
(PLOS, 2014-10-13)Tomato ABSCISIC ACID RIPENING 1 (ASR1) was the first cloned plant ASR gene. ASR orthologs were then cloned from a large number of monocot, dicot and gymnosperm plants, where they are mostly involved in response to abiotic ... -
Identification of candidate genes associated with leaf senescence in cultivated sunflower (Helianthus annuus L.)
(PLOS, 2014)Cultivated sunflower (Helianthus annuus L.), an important source of edible vegetable oil, shows rapid onset of senescence, which limits production by reducing photosynthetic capacity under specific growing conditions. ... -
Crop metabolomics : from diagnostics to assisted breeding
(Springer, 2018-11)Background Until recently, plant metabolomics have provided a deep understanding on the metabolic regulation in individual plants as experimental units. The application of these techniques to agricultural systems subjected ... -
Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism
(Wiley; Society for Experimental Biology, 2014-03)Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a ... -
Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
(Springer, 2019-04)Introduction: To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the ... -
In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
(Wiley, 2015-05)Epidemiological studies have shown that pollution derived from industrial and vehicular transportation induces adverse health effects causing broad ambient respiratory diseases. Therefore, air pollution should be taken ... -
Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model
(Elsevier, 2018-12)Paratuberculosis is a chronic disease caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease causes economic losses and, therefore, it is imperative to follow proper control strategies, which should include ... -
Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
(Elsevier, 2015-03)Bovine Anaplasmosis caused by Anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where Rhipicephalus microplus is considered the most significant biological vector. Molecular markers ... -
Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis
(Elsevier, 2015-12)Bovine tuberculosis (bTB) is a common zoonotic disease, caused by Mycobacterium bovis (M. bovis), responsible for significant economic losses worldwide. Its diagnosis is based on the detection of cell mediated immunity ...