Envíos recientes 

Instituto de Biotecnología: Envíos recientes

Mostrando ítems 181-200 de 437

    • Draft genome sequence of Mycobacterium bovis 04-303, a highly virulent strain from Argentina 

      Nishibe, Christiane; Canevari Castelão, Ana Beatriz; Dalla Costa, Ricardo; Pinto, Beatriz Jeronimo; Varuzza, Leonardo; Cataldi, Angel Adrian; Bernardelli, Amelia; Bigi, Fabiana; Blanco, Federico Carlos; Zumarraga, Martin Jose; Almeida, Nalvo Franco; Araujo, Flabio Ribeiro de (American Society for Microbiology, 2013-11)
      Mycobacterium bovis strain 04-303 was isolated from a wild boar living in a free-ranging field in Argentina. This work reports the draft genome sequence of this highly virulent strain and the genomic comparison of its major ...

    • Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts 

      Morcillo, Nora; Imperiale, Belen Rocio; Di Giulio, Angela Beatríz; Zumarraga, Martin Jose; Takif, Howard; Cataldi, Angel Adrian (Elsevier, 2014-12)
      There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this ...

    • Pre-columbian mycobacterial genomes reveal seals as a source of new world human tuberculosis 

      Bos, Kirsten I.; Harkins, Kelly M.; Herbig, Alexander; Coscolla, Mireia; Weber, Nico; Comas, Iñaki; Forrest, Stephen A.; Bryant, Josephine M.; Harris, Simon R.; Schuenemann, Verena J.; Campbell, Tessa J.; Majander, Kerrtu; Wilbur, Alicia K.; Guichon, Ricardo A.; Steadman, Dawnie L. Wolfe; Cook, Della Collins; Niemann, Stefan; Behr, Marcel A.; Zumarraga, Martin Jose; Bastida, Ricardo; Huson, Daniel; Nieselt, Kay; Young, Douglas; Parkhill, Julian; Buikstra, Jane E.; Gagneux, Sebastien; Stone, Anne C.; Krause, Johannes (Springer Nature, 2014-08)
      Modern strains of Mycobacterium tuberculosis from the Americas are closely related to those from Europe, supporting the assumption that human tuberculosis was introduced post-contact1. This notion, however, is incompatible ...

    • Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis 

      Blanco, Federico Carlos; Bianco, María Veronica; Garbaccio, Sergio Gabriel; Meikle, Virginia; Gravisaco, María José; Montenegro, Valeria Noely; Alfonseca, Edgar; Singh, Mahavir G.; Barandiaran, Soledad; Canal, Ana María; Vagnoni, Lucas Emilio; Buddle, Bryce Malcom; Bigi, Fabiana; Cataldi, Angel Adrian (Elsevier, 2013-05)
      A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per ...

    • Mycobacterioses in dogs and cats from Buenos Aires, Argentina 

      Barandiaran, Soledad; Martinez Vivot, Marcela; Falzoni, Elvira; Marfil, Maria Jimena; Perez Tort, Gabriela; Rovatti, Paula; Fernandez, Mónica; Iachini, Ricardo; Satek, Fernanda; Duchene, Adriana; Zumarraga, Martin Jose (Sage Publications, 2017-09)
      Mycobacterioses can produce nonspecific clinical signs in dogs and cats that make diagnosis difficult. Furthermore, the full characterization of mycobacterial agents is not always possible or practical. We characterized ...

    • Bovine tuberculosis in domestic pigs: Genotyping and distribution of isolates in Argentina 

      Barandiaran, Soledad; Martinez Vivot, Marcela; Pérez, Andrés M.; Cataldi, Angel Adrian; Zumarraga, Martin Jose (Elsevier, 2015-12)
      Bovine tuberculosis is caused by Mycobacterium bovis and affects primarily cattle, among many other mammal species. In this study, 250 isolates of M. bovis collected from pigs slaughtered in Argentina were typed by ...

    • Tuberculosis in swine co-infected with Mycobacterium avium subsp. hominissuis and Mycobacterium bovis in a cluster from Argentina 

      Barandiaran, Soledad; Pérez, Andrés M.; Gioffre, Andrea; Martinez Vivot, Marcela; Cataldi, Angel Adrian; Zumarraga, Martin Jose (Cambridge University Press, 2015-04)
      In Argentina little is known about the epidemiology of tuberculosis (TB) infection in swine. We characterized the epidemiological dynamics of Mycobacterium avium complex (MAC) infection in a swine population of Argentina ...

    • A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis 

      Trangoni, Marcos David; Gioffre, Andrea; Cravero, Silvio Lorenzo Pedro (Springer, 2017-06)
      LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, ...

    • Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversio 

      Ghio, Silvina; Bradanini, María B.; Garrido, Mercedes Maria; Ontañon, Ornella Mailen; Piccinni, Florencia Elizabeth; Marrero Diaz De Vill, Rubén; Talia, Paola Mónica; Campos, Eleonora (Elsevier, 2020-12)
      In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase ...

    • Development, characterization and experimental validation of a cultivated sunflower (Helianthus annuus L.) gene expression oligonucleotide microarray 

      Fernandez, Paula Del Carmen; Soria, Marcelo Abel; Blesa, David; Di Rienzo, Julio A.; Moschen, Sebastian Nicolas; Rivarola, Maximo Lisandro; Clavijo, Bernardo; Gonzalez, Sergio; Peluffo, Lucila; Principi, Dario; Dosio, Guillermo; Aguirrezabal, Luis; García-García, Francisco; Conesa, Ana; Hopp, Horacio Esteban; Dopazo, Joaquín; Heinz, Ruth Amelia; Paniego, Norma Beatriz (PLOS One, 2012-10)
      Oligonucleotide-based microarrays with accurate gene coverage represent a key strategy for transcriptional studies in orphan species such as sunflower, H. annuus L., which lacks full genome sequences. The goal of this study ...

    • SNP genotyping by heteroduplex analysis 

      Paniego, Norma Beatriz; Fusari, Corina Mariana; Lia, Veronica Viviana; Puebla, Andrea Fabiana (Springer, 2015-10)
      Heteroduplex-based genotyping methods have proven to be technologically effective and economically efficient for low- to medium-range throughput single-nucleotide polymorphism (SNP) determination. In this chapter we describe ...

    • Does Mycobacterium bovis persist in cattle in a non-replicative latent state as Mycobacterium tuberculosis in human beings? 

      Sabio Y Garcia, Julia Veronica; Bigi, María Mercedes; Klepp, Laura Ines; Garcia, Elizabeth Andrea; Blanco, Federico Carlos; Bigi, Fabiana (Elsevier, 2020-08)
      Members of the Mycobacterium tuberculosis complex (MTBC) are responsible for tuberculosis in several mammals. In this complex, Mycobacterium tuberculosis and Mycobacterium bovis, which are closely related, show host ...

    • A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness 

      Bei, Jinlong; Bigi, María Mercedes; Lima, Analía; Zhang, Qi; Blanco, Federico Carlos; López, Beatriz; Yu, Ting; Wang, Zhilin; Dai, Zhangyan; Chen, Zhuang; Cataldi, Angel Adrian; Sasiain, María del Carmen; Ritacco, Viviana; De la Barrera, Silvia Susana; Soria, Marcelo Abel; Durán, Rosario; Bigi, Fabiana (Hindawi, 2020-04)
      Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB ...

    • Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA‑seq analysis 

      Fass, Monica Irina; Rivarola, Maximo Lisandro; Ehrenbolger, Guillermo Federico; Maringolo, Carla Andrea; Montecchia, Juan Francisco; Quiroz, Facundo Jose; García‑García, Francisco; Dopazo Blázquez, Joaquín; Hopp, Horacio Esteban; Heinz, Ruth Amelia; Paniego, Norma Beatriz; Lia, Veronica Viviana (Springer Nature, 2020-08)
      Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance ...

    • The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? 

      Darqui, Flavia Soledad; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela (Crimson Publishers, 2020-08)
      Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been ...

    • Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis 

      Griffa, Natanael; Moyano, Roberto Damián; Canal, Ana María; Traveria, Gabriel Eduardo; Santangelo, María De La Paz; Alonso, Maria Natalia; Romano, Maria Isabel (Elsevier, 2020-02)
      Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving ...

    • Characterization of two GH5 endoglucanases from termite microbiome using synthetic metagenomics 

      Ben Guerrero, Emiliano; Marrero Diaz De Villegas, Ruben; Soria, Marcelo Abel; Santangelo, María De La Paz; Campos, Eleonora; Talia, Paola Mónica (Springer, 2020-08)
      Here, we characterize two novel GH5 endoglucanases (GH5CelA and GH5CelB) from an uncultured bacterium identified in termite gut microbiomes. Both genes were codon-optimized, synthetized, cloned, and expressed as recombinant ...

    • Development of diagnostic tools for IBDV detection using plants as bioreactors 

      Gomez, Evangelina Raquel; Cassani, María Florencia; Lucero, Maria Soledad; Parreño, Viviana; Chimeno Zoth, Silvina Andrea; Berinstein, Analia (SpringerOpen, 2020-05)
      Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric ...

    • Genomic re-assessment of the transposable element landscape of the potato genome 

      Zavallo, Diego; Crescente, Juan Manuel; Gantuz, Magdalena; Leone, Melisa; Vanzetti, Leonardo Sebastian; Masuelli, Ricardo Williams; Asurmendi, Sebastian (Springer, 2020-05)
      Transposable elements (TEs) are DNA sequences with the ability to autoreplicate and move throughout the host genome. TEs are major drivers in stress response and genome evolution. Given their significance, the development ...

    • Efficient method for targeted gene disruption by homologous recombination in Mycobacterium avium subspecie paratuberculosis 

      Alonso, Maria Natalia; Malaga, Wladimir; Mc Neil, Michael; Jackson, Mary; Romano, Maria Isabel; Guilhot, Christophe; Santangelo, María De La Paz (Elsevier, 2020-09)
      Targeted gene disruption by homologous recombination, has been widely used in mycobacterium species to understand the genetic basis of virulence and persistence in the host and to develop efficacious potential live vaccines. ...