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Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing
(Springer, 2022-07)The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ... -
Rapid and cost-effective process based on insect larvae for scale-up production of SARS-COV-2 spike protein for serological COVID-19 testing
(Wiley, 2021-10)Serology testing for COVID-19 is important in evaluating active immune response against SARS-CoV-2, studying the antibody kinetics, and monitoring reinfections with genetic variants and new virus strains, in particular, ... -
RdDM pathway components differentially modulate Tobamovirus symptom development
(Springer, 2020-08)Small RNAs (sRNAs) are important molecules for gene regulation in plants and play an essential role in plant-pathogen interactions. Researchers have evaluated the relationship between viral infections as well as the ... -
Recent advances in non-specific immune memory against bovine tuberculosis
(Elsevier, 2021-04)Bovine tuberculosis is an important worldwide disease mainly related to cattle, although it also affects other mammals, including humans. In recent years, there have been considerable advances in the knowledge of the immune ... -
Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
(2016)To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units ... -
Recombinant occlusion bodies of baculovirus as carriers of a nonstructural protein of foot-and-mouth disease virus
(Springer, 2018-11)Here, we developed a diagnostic ELISA for foot-and-mouth disease using recombinant occlusion bodies (rOBs) of baculovirus. We fused Δ3AB1–3, a polypeptide derived from non-structural proteins of foot-and-mouth disease ... -
Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers
(BioMed Central, 2011-12)Background: Planthoppers not only severely affect crops by causing mechanical damage when feeding but are also vectors of several plant virus species. The analysis of gene expression in persistently infected planthoppers ... -
Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
(Springer, 2022-05)Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant ... -
Relationships between bioactive compound content and the antiplatelet and antioxidant activities of six allium vegetable species
(2017)Allium sp. vegetables are widely consumed for their characteristic flavour. Additionally, their consumption may provide protection against cardiovascular disease due to their antiplatelet and antioxidant activities. Although ... -
Relevancia clínica, diversidad y variabilidad genética de distintas especies del género Mycobacterium
(2017)Introducción: El término micobacterias no tuberculosas (MNT) incluye distintas especies ambientales capaces de enfermar humanosy/o animales incluso mediante una probable transmisión zoonótica Objetivos. Determinar: la ... -
Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model
(Elsevier, 2022-05)Cattle vaccination is an attractive approach in compliance with control and eradication programs against Bovine Tuberculosis (bTB). Today, there is no anti bTB vaccine licensed. Two vaccine candidates, MbΔmce2 and MbΔmce2-phoP ... -
Research Note : Characterization and phylodynamic analysis of new infectious bursal disease virus variants circulating in Argentina
(Elsevier, 2024-06)Infectious Bursal Disease is a highly contagious disease that affects young chickens and leads to significant economic losses. Its causal agent is a double-stranded RNA virus that, due to its high error rate during the ... -
Respuesta inmune en terneros experimentalmente infectados con diferentes dosis de Mycobacterium bovis
(Gerencia de Comunicación e Imagen Institucional, DNA SICC, INTA, 2011-08)La tuberculosis bovina es una enfermedad zoonótica producida por Mycobacterium bovis que afecta a animales de producción causando pérdidas económicas y representa, además, un problema para la salud pública. El conocimiento ... -
Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
(Springer, 2022-07)The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities ... -
Role of PhoPR in the response to stress of Mycobacterium bovis
(Elsevier, 2021-02)PhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative ... -
Rv2577 of Mycobacterium tuberculosis is a virulence factor with dual phosphatase and phosphodiesterase functions
(Frontiers Media, 2020-10)Tuberculosis, a lung disease caused by Mycobacterium tuberculosis (Mtb), is one of the ten leading causes of death worldwide affecting mainly developing countries. Mtb can persist and survive inside infected cells through ... -
Rv2617c and P36 are virulence factors of pathogenic mycobacteria involved in resistance to oxidative stress
(Taylor & Francis, 2019-12)In this study, we characterized the role of Rv2617c in the virulence of Mycobacterium tuberculosis. Rv2617c is a protein of unknown function unique to M. tuberculosis complex (MTC) and Mycobacterium leprae. In vitro, this ... -
Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes
(2013-12)Losses produced by virus diseases depend mostly on symptom severity. Turnip mosaic virus (TuMV) is one of the most damaging and widespread potyvirus infecting members of the family Brassicaceae, including Arabidopsis ... -
SARS-CoV-2 RBD protein enhances the oncolytic activity of the vesicular stomatitis virus
(Frontiers Media, 2023-01)Despite recent advances in the research on oncolytic viruses (OVs), a better understanding of how to enhance their replication is key to improving their therapeutic index. Understanding viral replication is important to ... -
Secretion systems of pathogenic escherichia coli
(Springer International Publishing, 2016)Protein secretion plays a central role in modulating the interactions of bacteria with their environments. Bacterial ribosomes synthesize up to 8000 different proteins. Almost half of these become integrated in membranes ...