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Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
(Springer, 2018-08)As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand ... -
Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
(BMC Plant Biology, 2014-09-17)Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. ... -
CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa
(Springer, 2023-02)Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying ... -
Efficient CRISPR/Cas9 genome editing in alfalfa using a public germplasm
(Frontiers Media, 2021-07)Because its ability to acquire large amounts of nitrogen by symbiosis, tetraploid alfalfa is the main source of vegetable proteins in meat and milk production systems in temperate regions. Alfalfa cultivation also adds ... -
Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa
(Frontiers Media, 2022-04)Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research ... -
Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia
(Springer, 2022-02)We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present ... -
Generation of a multi‑herbicide‑tolerant alfalfa by using base editing
(Springer, 2022-02)We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important ... -
pBAR–H3.2, a native-optimized binary vector to bypass transgene silencing in alfalfa
(Springer, 2020-03)Alfalfa is the main forage worldwide due to its high biomass production, excellent nutritional qualities and adaptation to a wide range of environments (Singer et al. 2018). Besides, due to its ability to grow without ... -
Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
(Oxford University Press, 2015-01)Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. ... -
Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing
(Springer, 2022-07)The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ... -
Spontaneous Mutations in the Nitrate Reductase Gene napC Drive the Emergence of Eco-friendly Low-N2O-Emitting Alfalfa Rhizobia in Regions with Different Climates
(Springer, 2020)We have recently shown that commercial alfalfa inoculants (e.g., Sinorhizobium meliloti B399), which are closely related to the denitrifier model strain Sinorhizobium meliloti 1021, have conserved nitrate, nitrite, and ... -
Subfunctionalization probably drives the emergence of plant growth‑promoting genes
(Springer, 2022-07)The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution ... -
The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa
(Springer, 2022-12)The CRISPR/Cas9 system has proven to be highly valuable for genome editing in several important crops, including the allogamous tetraploid cultivated alfalfa (Medicago sativa). However, the fact that most of the beneficial ...