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A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
(Springer, 2017-06)LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, ... -
Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
(Scientific Research, 2012-02)Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but ... -
Estudio de la variabilidad genética de Brucella melitensis en el noroeste de la provincia de San Luis
(Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 2020)Los objetivos del presente trabajo fueron emplear técnicas de genotipificación molecular en un escenario real, describir genotipos circulantes de Brucella melitensis en el noroeste de la provincia de San Luis y poner a ... -
A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats
(2017-08)Brucellosis is the leading zoonosis on a worldwide scale and constitutes a major public health threat in many regions of the world. Several molecular markers associated with natural resistance to intracellular bacterial ... -
Pathogenesis and immunobiology of brucellosis : review of brucella-host interactions
(Elsevier, 2015-06)This review of Brucellaehost interactions and immunobiology discusses recent discoveries as the basis for pathogenesis-informed rationales to prevent or treat brucellosis. Brucella spp., as animal pathogens, cause human ... -
Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
(Elsevier, 2014-08-15)Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) ... -
Use of Chelex-100 for the molecular diagnosisof five animal pathogens
(Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2021-06)Molecular tools have improved conventional veterinary diagnosis. Acid nucleic extraction is a key step for downstream applications. This work aimed to compare the DNA extraction method Chelex-100 resin (M1) ...