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Mostrando ítems 1-10 de 10
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CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa
Galindo Sotomonte, Luisa Fernanda; Jozefkowicz, Cintia; Gomez, Maria Cristina; Stritzler, Margarita; Frare, Romina Alejandra; Bottero, Ana Emilia; Tajima, Hiromi; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2023-02)Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying ... -
CRISPR/Cas9‑mediated knockout of SPL13 radically increases lettuce yield
Beracochea, Valeria Cecilia; Stritzler, Margarita; Radonic, Laura Mabel; Bottero, Ana Emilia; Jozefkowicz, Cintia; Darqui, Flavia Soledad; Ayub, Nicolás Daniel; Lopez Bilbao, Marisa Gisela; Soto, Gabriela Cynthia (Springer, 2022-12)Cultivated lettuce (Lactuca sativa L.) is a diploid (2n = 2x = 18), autogamous species. This popular leafy vegetable is considered one of the first crops (Guo et al. 2022). Currently, lettuce is the third most consumed ... -
Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa
Jozefkowicz, Cintia; Gomez, Maria Cristina; Odorizzi, Ariel; Iantcheva, Anelia; Ratet, Pascal; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Frontiers Media, 2022-04)Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research ... -
Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions
Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Puente, Mariana Laura; Piccinetti, Carlos Fabian; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-08)Despite the vast screening for natural nitrogenfixing isolates by public and private consortia, no significant progresses in the production of improved nitrogenfixing inoculants for alfalfa production have been made in the ... -
Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
Bottero, Ana Emilia; Pascuan, Cecilia Gabriela; Pagano, Elba Maria; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia; Jozefkowicz, Cintia (2016)Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the ... -
Pip water transport and its pH dependence are regulated by tetramer stoichiometry
Jozefkowicz, Cintia; Sigaut, Lorena; Scochera, Florencia; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel; Pietrasanta, Lía Isabel; Amodeo, Gabriela; Gonzalez Flecha, Francisco Luis; Alleva, Karina Edith (2016)Many plasma membrane channels form oligomeric assemblies, and heterooligomerization has been described as a distinctive feature of some protein families. In the particular case of plant plasma membrane aquaporins (PIPs), ... -
Spontaneous Mutations in the Nitrate Reductase Gene napC Drive the Emergence of Eco-friendly Low-N2O-Emitting Alfalfa Rhizobia in Regions with Different Climates
Brambilla, Silvina Maricel; Soto, Gabriela; Odorizzi, Ariel; Arolfo, Valeria; McCormick, Wayne; Primo, Emiliano; Giordano, Walter; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Springer, 2020)We have recently shown that commercial alfalfa inoculants (e.g., Sinorhizobium meliloti B399), which are closely related to the denitrifier model strain Sinorhizobium meliloti 1021, have conserved nitrate, nitrite, and ... -
Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication
Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Piccinetti, Carlos Fabian; Puente, Mariana Laura; Berini, Carolina Andrea; Reyes Perez, Pedro Jose; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-12)We here characterized the stress-tolerant alfalfa microsymbiont Sinorhizobium meliloti B401. B401-treated plants showed high nitrogen fixation rates under humid and semiarid environments. The production of glycine betaine ... -
Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils
Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Soto, Gabriela Cynthia; Berini, Carolina Andrea; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Elsevier, 2020-09)Objectives: Unlike higher organisms such as domestic animals and cultivated plants, which display a robust reproductive isolation and limited dispersal ability, microbes exhibit an extremely promiscuous gene flow and can ... -
The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa
Gomez, Maria Cristina; Jozefkowicz, Cintia; Mozzicafreddo, Matteo; Odorizzi, Ariel; Arolfo, Valeria; Basigalup, Daniel Horacio; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-12)The CRISPR/Cas9 system has proven to be highly valuable for genome editing in several important crops, including the allogamous tetraploid cultivated alfalfa (Medicago sativa). However, the fact that most of the beneficial ...